中脑星形胶质细胞源性神经营养因子在人、大鼠及小鼠脑中的表达分布

唐小露; 邓聪聪; 汪佩; 陈明辉; 田楷; 苏彦艳; 马惠; 吴雪艳; 沈玉先

doi:10.16098/j.issn.0529-1356.2022.05.002

解剖学报 >

2022 , Vol. 53 >Issue 5: 551 - 556

DOI: https://doi.org/10.16098/j.issn.0529-1356.2022.05.002

神经生物学

中脑星形胶质细胞源性神经营养因子在人、大鼠及小鼠脑中的表达分布

唐小露 ,
邓聪聪 ,
汪佩 ,
陈明辉 ,
田楷 ,
苏彦艳 ,
马惠 ,
吴雪艳 ,
沈玉先

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1.安徽医科大学基础医学院人体解剖学教研室，合肥 230032; 2.安徽医科大学第二临床医学院临床医学专业2017级，合肥 230032; 3.安徽医科大学第一临床医学院临床医学专业2017级，合肥 230032; 4.安徽医科大学第二临床医学院临床医学专业2018级，合肥 230032; 5.安徽医科大学第一临床医学院临床医学专业2016级，合肥 230032; 6. 安徽医科大学生物药物研究所，合肥 230032; 7.安徽医科大学基础医学院，合肥 230032

收稿日期: 2022-03-04

修回日期: 2022-04-15

网络出版日期: 2022-10-06

基金资助

安徽医科大学博士科研资助项目;安徽省自然科学基金资助项目;国家重点研发计划

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Distribution of mesencephalic astrocyte derived neurotrophic factor in human, mouse and rat brain

TANG Xiao-Lu ,
DENG Cong-Cong ,
WANG Pei ,
CHEN Ming-Hui ,
TIAN Kai ,
SU Yan-Yan ,
MA Hui ,
WU Xue-Yan ,
SHEN Yu-Xian

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1.Department of Human Anatomy, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China;2.Grade of 2017, Majoring in Clinical Medicine, the Second Clinical Medical College, Anhui Medical University, Hefei 230032, China; 3.Grade of 2017, Majoring in Clinical Medicine, the First Clinical Medical College, Anhui Medical University, Hefei 230032, China; 4.Grade of 2018, Majoring in Clinical Medicine, the Second Clinical Medical College of Anhui Medical University, Hefei 230032, China; 5.Grade of 2016, Majoring in Clinical Medicine, the First Clinical Medical College, Anhui Medical University, Hefei 230032, China; 6.Biopharmaceutical Research Institute, Anhui Medical University, Hefei 230032, China;7,School of Basic Medical Sciences, Anhui Medical University, Hefei 20032, China

Received date: 2022-03-04

Revised date: 2022-04-15

Online published: 2022-10-06

Supported by

The Doctoral Research Funding Project at Anhui Medical University;The Anhui Natural Science Foundation;The National Key R&D Program of China

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摘要

目的探讨中脑星形胶质细胞源性神经营养因子(MANF)在人、大鼠和小鼠脑中的表达分布。方法使用免疫组织化学染色方法，在5例人脑标本、6只小鼠及6只大鼠中检测MANF在人、大鼠和小鼠脑中的表达分布情况并比较物种间差异性。结果 MANF在人、大鼠及小鼠脑中的表达水平具有脑区差异性。在人脑中，MANF在大脑皮层的表达水平最高，在皮层下区域，脑干和小脑的表达水平稍弱。MANF在大鼠和小鼠脑内的表达分布与人脑略有不同，但总体一致。结论 MANF在人、大鼠和小鼠脑中的表达情况大致相同，但在物种内部，MANF在不同脑区中表达水平存在差异。

关键词： 中脑星形胶质细胞源性神经营养因子; 脑区分布; 免疫组织化学; 人; 大鼠; 小鼠

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唐小露 , 邓聪聪 , 汪佩 , 陈明辉 , 田楷 , 苏彦艳 , 马惠 , 吴雪艳 , 沈玉先 . 中脑星形胶质细胞源性神经营养因子在人、大鼠及小鼠脑中的表达分布[J]. 解剖学报, 2022 , 53(5) : 551 -556 . DOI: 10.16098/j.issn.0529-1356.2022.05.002

Abstract

Objective To observe the distribution of mesencephalic astrocyte derived neurotrophic factor (MANF) within the brain in human, mice and rats and the comparison across the species. Methods An immunohistochemical method was applied to investigate the expression of MANF and to compare the differences among species in the brain of human specimens (n=5), mice (n=6), and rats (n=6). Results The expression of MANF varied in different brain regions in human, mice and rats. In human, in the cortex, the expression of MANF was the highest among all brain regions. In the subcortical areas or the nucleus, brainstem and cerebellum expression level of MANF were relatively lower. The distribution of MANF in mouse and rat brain were slightly different from that in human brain, but generally consistent. Conclusion The distribution pattern of MANF is similar across the species, however, within a species, MANF expression levels varies in different brain regions.

Key words： Mesencephalic astrocyte derived neurotrophic factor; Brain distribution; Immunohistochemistry; Human; Rat; Mouse

参考文献

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向春晨, 韩芳, 石玉秀. 内质网应激中活化转录因子6通路及相关调控因子对未折叠蛋白反应的调控［J］. 解剖学报, 2015, 46(6): 857-861.

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