中脑星形胶质细胞源性神经营养因子在人、大鼠及小鼠脑中的表达分布

唐小露; 邓聪聪; 汪佩; 陈明辉; 田楷; 苏彦艳; 马惠; 吴雪艳; 沈玉先

doi:10.16098/j.issn.0529-1356.2022.05.002

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解剖学报 ›› 2022, Vol. 53 ›› Issue (5) : 551-556. DOI: 10.16098/j.issn.0529-1356.2022.05.002

神经生物学

中脑星形胶质细胞源性神经营养因子在人、大鼠及小鼠脑中的表达分布

唐小露¹ 邓聪聪² 汪佩¹ 陈明辉³ 田楷⁴ 苏彦艳¹ 马惠⁵ 吴雪艳^1* 沈玉先^6,7*

作者信息 +

Distribution of mesencephalic astrocyte derived neurotrophic factor in human, mouse and rat brain

TANG Xiao-lu¹ DENG Cong-cong² WANG Pei¹ CHEN Ming-hui³ TIAN Kai⁴ SU Yan-yan¹ MA Hui⁵ WU Xue-yan^1* SHEN Yu-xian^6,7*

Author information +

文章历史 +

摘要

目的探讨中脑星形胶质细胞源性神经营养因子(MANF)在人、大鼠和小鼠脑中的表达分布。方法使用免疫组织化学染色方法，在5例人脑标本、6只小鼠及6只大鼠中检测MANF在人、大鼠和小鼠脑中的表达分布情况并比较物种间差异性。结果 MANF在人、大鼠及小鼠脑中的表达水平具有脑区差异性。在人脑中，MANF在大脑皮层的表达水平最高，在皮层下区域，脑干和小脑的表达水平稍弱。MANF在大鼠和小鼠脑内的表达分布与人脑略有不同，但总体一致。结论 MANF在人、大鼠和小鼠脑中的表达情况大致相同，但在物种内部，MANF在不同脑区中表达水平存在差异。

Abstract

Objective To observe the distribution of mesencephalic astrocyte derived neurotrophic factor (MANF) within the brain in human, mice and rats and the comparison across the species. Methods An immunohistochemical method was applied to investigate the expression of MANF and to compare the differences among species in the brain of human specimens (n=5), mice (n=6), and rats (n=6). Results The expression of MANF varied in different brain regions in human, mice and rats. In human, in the cortex, the expression of MANF was the highest among all brain regions. In the subcortical areas or the nucleus, brainstem and cerebellum expression level of MANF were relatively lower. The distribution of MANF in mouse and rat brain were slightly different from that in human brain, but generally consistent. Conclusion The distribution pattern of MANF is similar across the species, however, within a species, MANF expression levels varies in different brain regions.

导出引用

唐小露邓聪聪汪佩陈明辉田楷苏彦艳马惠吴雪艳沈玉先. 中脑星形胶质细胞源性神经营养因子在人、大鼠及小鼠脑中的表达分布[J]. 解剖学报. 2022, 53(5): 551-556 https://doi.org/10.16098/j.issn.0529-1356.2022.05.002

TANG Xiao-lu DENG Cong-cong WANG Pei CHEN Ming-hui TIAN Kai SU Yan-yan MA Hui WU Xue-yan SHEN Yu-xian. Distribution of mesencephalic astrocyte derived neurotrophic factor in human, mouse and rat brain[J]. Acta Anatomica Sinica. 2022, 53(5): 551-556 https://doi.org/10.16098/j.issn.0529-1356.2022.05.002

中图分类号： Ｒ322. 81

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