目的  探讨氨酰转运RNA合成酶复合物相互作用多功能蛋白 1 (AIMP1) 的N端25多肽链（α-ANT）对皮肤损伤后瘢痕形成和愈合过程的作用。方法  利用无菌手术刀或9 mm皮肤打孔器在SPF级CD-1雄性小鼠（n=51）中分别构建全层切口伤口或全层切除伤口2个模型。全层切口伤口模型制作后分为单纯全层切口伤口模型组（空白组，n=9），全层切口伤口模型+ Pluronic凝胶载体组（n=9）及全层切口伤口模型+α-ANT治疗组（60 μmol/L，n=12）；全层切除伤口制作后分为全层切除伤口模型+ Pluronic凝胶载体组（对照组，n=9）及全层切除伤口模型+α-ANT治疗组（60 μmol/L，n=12）。评估伤口愈合程度，HE染色观察肉芽组织病理学变化，Western blotting及免疫荧光检测炎症细胞因子和生长因子表达水平；多功能强力仪检测皮损修复后的拉伸性能；CCK-8和Transwell趋化实验分别检测α-ANT对原代分离中性粒细胞增殖、迁移特性的影响。结果  通过伤口涂抹方式进行外源性α-ANT肽的补充后，α-ANT肽在小鼠全层皮肤创面模型建立后2 h即可富集于伤口边缘。无论是线形（切割伤）、圆形（穿刺伤）伤口，应用α-ANT肽均显著加速创面愈合，减少瘢痕组织生成，同时有效促进皮损愈合后真皮组织结构和机械强度的恢复。Western blotting及免疫荧光结果显示，α-ANT肽给增强ERK 活化，抑制P38 MAPK活化，减少中性粒细胞浸润，增强生长因子的合成。结论  无论皮肤伤口形态如何，α-ANT多肽均可通过调控ERK-P38 MAPK的动态平衡抑制炎症过程中性粒细胞浸润和分泌，显著促进皮肤创面愈合。

Objective  To examine the impact of a 25-amino-acid N-terminal peptide (α-ANT) derived from aminoacyl-transfer RNA synthetase complex-interacting multifunctional protein 1 (AIMP1) on scar development and the process of wound healing following skin injury. Methods  The full-thickness incision wound or full-thickness excision wound models were constructed in SPF-grade CD-1 male mice (n=51) using a sterile scalpel or a 9 mm skin punch. After creating the full-thickness incision wound model, the mice were divided into the simple full-thickness incision wound model group (blank group, n=9), the full-thickness incision wound model + Pluronic gel group (n=9), and the full-thickness incision wound model + α-ANT treatment group (60 μmol/L, n=12). After creating the full-thickness excision wound model, the mice were divided into the full-thickness excision wound model + Pluronic gel group (control group, n=9) and the full-thickness excision wound model + α-ANT treatment group (60 μmol/L, n=12). Wound healing was assessed, and HE staining was used to observe pathological changes in granulation tissue. Western blotting and immunofluorescence were employed to measure the expression levels of inflammatory cytokines and growth factors. The tensile properties of the skin lesions after repair were evaluated using a multifunctional tensile tester. CCK-8 and Transwell chemotaxis assays were conducted to examine the effects of α-ANT on the proliferation and migration characteristics of primary neutrophils. Results   After topical application of exogenous α-ANT peptide, the peptide could accumulate at the wound edges within 2 hours following the establishment of full-thickness skin wound models in mice. Whether applied to linear (incisional) or circular (puncture) wounds, α-ANT peptide significantly accelerated wound healing, reduced scar tissue formation, and effectively promoted the restoration of dermal tissue structure and mechanical strength post-healing. Western blotting and immunofluorescence results demonstrated that α-ANT peptide enhanced ERK activation, inhibited P38 MAPK activation, reduced neutrophil infiltration, and stimulated growth factor synthesis. Conclusion  Regardless of the morphology of skin wounds, α-ANT peptides can significantly promote skin wound healing by modulating the dynamic balance of ERK-P38 MAPK to inhibit neutrophil infiltration and secretion during the inflammatory process.