T细胞免疫球蛋白黏蛋白3高表达促进单核细胞迁移预测非小细胞肺癌预后

ZHOU  Lu1  CHEN  Si-tong2  LI  Chun-yan3  HUANG  Yan-yan3  XU  Li-yun1; 3*

doi:10.16098/j.issn.0529-1356.2025.06.009

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解剖学报 ›› 2025, Vol. 56 ›› Issue (6) : 697-703. DOI: 10.16098/j.issn.0529-1356.2025.06.009

肿瘤生物学

T细胞免疫球蛋白黏蛋白3高表达促进单核细胞迁移预测非小细胞肺癌预后

周璐¹ 陈思通² 李春燕³ 黄燕燕³ 续力云^1*

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High expression of T-cell immunoglobulin and mucin domain-containing protein 3 predicting prognosis of non-small cell lung cancer by promoting monocyte migration

ZHOU Lu¹ CHEN Si-tong² LI Chun-yan³ HUANG Yan-yan³ XU Li-yun^1,3*

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摘要

目的探讨T细胞免疫球蛋白黏蛋白3（TIM-3）在外周血单核细胞中的表达，及其与非小细胞肺癌（NSCLC）之间的关系。方法采用流式细胞术分析116例NSCLC患者和37例健康志愿者外周血单核细胞TIM-3表达。多重免疫组织化学检测组织巨噬细胞TIM-3的表达。细胞迁移实验检测单核细胞迁移能力。采用Real-time PCR和ELISA检测单核细胞TIM-3 mRNA和蛋白表达。结果与健康志愿者相比，NSCLC患者外周血单核细胞TIM-3表达上调(P<0.05)。NSCLC患者TIM-3主要在癌旁组织巨噬细胞表达，与单核细胞表达成正相关（P<0.05）。TIM-3高表达促进NSCLC患者单核细胞迁移（P<0.05），外周血单核细胞TIM-3高表达预示预后不良（P<0.05）。结论外周血单核细胞TIM-3表达可能是预测NSCLC患者预后的一个有用指标，TIM-3可能成为免疫治疗策略的新靶点。

Abstract

Objective To investigate the expression of T cell immunoglobulin and mucin domaincontaining protein 3 (TIM-3) in peripheral blood mononuclear cells and its relationship with non-small cell lung cancer (NSCLC). Methods TIM-3 expression in monocytes from the peripheral blood of 116 NSCLC patients and 37 healthy volunteers was analyzed via flow cytometry. The expression of TIM-3 in macrophages from tissues was detected via multiplex immunohistochemical staining. Transwell migration experiments were used to detect the migration ability of monocytes. Real-time PCR and ELISA were used to detect the mRNA and protein expression of TIM-3 in monocytes. Results Compared with that in healthy volunteers, TIM-3 expression was upregulated in the peripheral blood monocytes of NSCLC patients (P<0.05). TIM-3 was expressed mainly in macrophages in adjacent tissues and positively correlated with its expression in monocytes in NSCLC patients (P<0.05). Furthermore, high TIM-3 expression promoted monocyte migration in NSCLC patients (P<0.05), and high TIM-3 expression in peripheral blood monocytes indicated poor prognosis(P<0.05). Conclusion TIM-3 expression in peripheral blood monocytes may be a useful indicator of the disease prognosis of NSCLC patients and TIM-3 could be a new target for immunotherapeutic strategies.

关键词

T细胞免疫球蛋白黏蛋白3 / 单核细胞 / 迁移能力 / 非小细胞肺癌 / 预后 / 流式细胞术 / 人

Key words

T cell immunoglobulin and mucin domain-containing protein 3

/ Monocyte / Migration ability / Non-small cell lung cancer / Prognosis / Flow cytometry / Human

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周璐1 陈思通李春燕黄燕燕续力云. T细胞免疫球蛋白黏蛋白3高表达促进单核细胞迁移预测非小细胞肺癌预后[J]. 解剖学报. 2025, 56(6): 697-703 https://doi.org/10.16098/j.issn.0529-1356.2025.06.009

ZHOU Lu CHEN Si-tong LI Chun-yan HUANG Yan-yan XU Li-yun.

High expression of T-cell immunoglobulin and mucin domain-containing protein 3 predicting prognosis of non-small cell lung cancer by promoting monocyte migration

[J]. Acta Anatomica Sinica. 2025, 56(6): 697-703 https://doi.org/10.16098/j.issn.0529-1356.2025.06.009

中图分类号： R329.2

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