膜联蛋白A2和糖原合成酶激酶-3β在皮肤鳞状细胞癌中的表达及临床意义

doi:10.16098/j.issn.0529-1356.2025.03.006

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解剖学报 ›› 2025, Vol. 56 ›› Issue (3) : 294-300. DOI: 10.16098/j.issn.0529-1356.2025.03.006

肿瘤生物学

膜联蛋白A2和糖原合成酶激酶-3β在皮肤鳞状细胞癌中的表达及临床意义

蒋文群¹ 侯品品²谭美乐³ 甘林权³ 吴玲艳³ 成先桂^3*

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Expression and clinical significance of annexin A2 and glycogen synthase kinase-3β in cutaneous squamous cell carcinoma

JIANG Wen-qun¹ HOU Pin-pin² TAN Mei-le³ GAN Lin-quan³ WU Ling-yan³ CHENG Xian-gui^3*

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摘要

目的探讨膜联蛋白A2（annexin A2）和糖原合成酶激酶-3β（GSK-3β）在皮肤鳞状细胞癌（CSCC）组织中的表达，分析其与CSCC的相关性和临床病理诊断价值。方法收集2020年10月~2024年5月在南宁市第二人民医院皮肤科接受手术治疗的68例CSCC患者和40例角化棘皮瘤（KA）患者的病理组织，并以32例良性皮肤病患者的周边正常皮肤组织作为对照。采用免疫组织化学法和Western blotting检测3组annexin A2、GSK-3β及β-连环蛋白（β-catenin）的蛋白表达。采用Spearman评估CSCC癌组织annexin A2和GSK-3β表达与肿瘤病理特征之间的相关性。绘制受试者操作特征曲线（ROC curve），分析annexin A2和GSK-3β在CSCC临床病理诊断中的价值。结果与正常皮肤组织相比，CSCC癌组织annexin A2和β-catenin表达升高，GSK-3β表达降低（P＜0.05）；与KA组织相比，CSCC癌组织annexin A2表达升高（P＜0.05）。CSCC癌组织annexin A2与GSK-3β表达成负相关（r=-0.3901，P<0.01）。GSK-3β与组织分化程度相关，低分化患者癌组织GSK-3β表达较低（P＜0.05）。Annexin A2和GSK-3β诊断CSCC的敏感度分别为85.3％和41.2％，特异度分别为46.9%和84.4％；Annexin A2鉴别诊断CSCC与KA的敏感度为85.3％，特异度为40.0％。结论 AnnexinA2和GSK-3β可作为CSCC的早期诊断或鉴别诊断的潜在生物标志物，在CSCC发生发展中发挥着重要作用，其作用机制可能与Wnt/β-catenin信号通路的激活有关。

Abstract

Objective To investigate the expressions of annexin A2 and glycogen synthesis kinase-3β (GSK-3β) in cutaneous squamous cell carcinoma (CSCC) tissues, and to analyze their correlation with CSCC as well as their clinical pathological diagnostic value. Methods The pathological tissues of 68 patients with CSCC and 40 patients with keratoacanthoma (KA) who underwent surgical treatment in the Department of Dermatology of the Second Hospital of Nanning from October 2020 to May 2024 were collected, and the surrounding normal skin tissues of 32 patients with benign skin diseases were used as controls. The expressions of annexin A2, GSK-3β and β-catenin were detected by immunohistochemistry and Western blotting. Spearman was used to evaluate the correlation between the expressions of annexin A2 and GSK-3β and the pathological characteristics in CSCC. The receiver operating characteristic (ROC) curve was drawn to analyze the clinical diagnostic value of annexin A2 and GSK-3β in CSCC. Results Compared with the normal skin tissues, the expressions of annexin A2 and βcatenin in CSCC increased, and GSK-3β decreased (P<0.05); Compared with the KA tissues, the expression of annexin A2 in CSCC tissues increased (P<0.05). The expression of annexin A2 was negatively correlated with that of GSK-3β in CSCC (r=-0.3901, P<0.01). GSK-3β expression was related to tissue differentiation, with lower expression in poorly differentiated patients’ cancer tissues (P<0.05). The sensitivity of annexin A2 and GSK-3β for diagnosis of CSCC was 85.3% and 41.2%, respectively, with specificities of 46.9% and 84.4% respectively. The sensitivity of annexin A2 for distinguishing between CSCC and KA was 85.3%, with a specificity of 40.0%. Conclusion Annexin A2 and GSK-3β may be used as potential biomarkers for the early diagnosis or differential diagnosis of CSCC, and play important roles in the development of CSCC. Their mechanism may be related to the activation of Wnt/β-catenin signaling pathway.

关键词

皮肤鳞状细胞癌

/ 角化棘皮瘤 / 膜联蛋白A2 / 糖原合成酶激酶-3β / 免疫印迹法 / 人

Key words

Cutaneous squamous cell carcinoma

/ Keratoacanthoma / Annexin A2 / Glycogen synthesis kinase-3β / Western blotting / Huma

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蒋文群侯品品谭美乐甘林权吴玲艳成先桂. 膜联蛋白A2和糖原合成酶激酶-3β在皮肤鳞状细胞癌中的表达及临床意义[J]. 解剖学报. 2025, 56(3): 294-300 https://doi.org/10.16098/j.issn.0529-1356.2025.03.006

JIANG Wen-qun HOU Pin-pin TAN Mei-le GAN Lin-quan WU Ling-yan CHENG Xian-gui. Expression and clinical significance of annexin A2 and glycogen synthase kinase-3β in cutaneous squamous cell carcinoma[J]. Acta Anatomica Sinica. 2025, 56(3): 294-300 https://doi.org/10.16098/j.issn.0529-1356.2025.03.006

中图分类号： R322.99 R739.5

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