目的 探讨CHD8^+/-小鼠的行为模式和海马神经发生的变化情况，为研究改善CHD8^+/-小鼠自闭样行为和海马神经发生提供行为学和形态基础。 方法 野生型（WT）和CHD8^+/-小鼠基因鉴定。雄、雌性WT和CHD8^+/-小鼠称重，进行3箱社交, 自我捋毛, 筑巢, Y迷宫自发交替, 食物埋藏, 旷场和黑白箱行为学实验。2%戊巴比妥钠（2 ml/kg）麻醉，4%多聚甲醛灌注，取脑，摄片，分析脑尺寸的变化；结果  Western blotting及免疫荧光染色检测海马新生神经元的变化。结果 Western blotting结果显示，雄、雌性CHD8^+/-小鼠的染色体域解旋酶DNA结合蛋白8（CHD8）蛋白水平均较WT小鼠减少。雄、雌性WT和CHD8^+/-小鼠体重和脑尺寸差异均无显著性。3箱社交行为实验显示，雄、雌性CHD8^+/-小鼠社交障碍（P＜0.05）；旷场实验结果显示，雄、雌性WT和CHD8^+/-小鼠活动总距离差异无显著性，但CHD8^+/-小鼠在旷场中心区域的停留时间较WT小鼠显著减少（P＜0.01）；黑白箱实验显示，雄、雌性CHD8^+/-小鼠在白箱内的探索时间较WT小鼠明显减少（P＜0.05）；而自我捋毛，筑巢，Y迷宫自发交替，食物埋藏实验均无显著变化。此外，Western blotting结果显示，CHD8^+/-小鼠海马区双肾上腺皮质激素（DCX）表达减少（P＜0.001），免疫荧光染色也显示，CHD8^+/-小鼠海马DCX⁺ 细胞数量减少（P＜0.01）。 结论 CHD8^+/-小鼠表现为社交障碍和焦虑样行为，海马新生神经元数量减少，神经发生障碍。

Objective  To explore the behavioral patterns and hippocampal neurogenesis of CHD8^+/- mice, and to provide behavioral and morphological basis for improving autism like behavior and neurogenesis.   Methods  Genotype of wild type (WT) and CHD8^+/- mice was identified. Weight measurement was conducted on both male and female mice of the WT and CHD8^+/- strains. Subsequently, a battery of behavioral tests was administered, which included three-chamber test, self-grooming test, nesting test, Y-maze spontaneous alternation test,  food burial test, open-field test and light-dark transition test. Afterwards, the mice were administered 2% pentobarbital sodium (2 ml/kg) to induce anesthesia. Their brains were frozen with 4% paraformaldehyde, removed for photography and analysis to identify any alterations in brain size. Western blotting and immunofluorescent labeling were used to detect changes in the process of hippocampus neurogenesis.   Results  Western blotting analysis demonstrated a decrease in the amounts of chromodomain helicase DNA binding protein 8 (CHD8) protein in both male and female mice with CHD8^+/- genotype, as compared to WT mice. There were no notable disparities in body weight between male and female WT and CHD8^+/- mice, as well as in brain size. The three-chamber social behavior test revealed that both male and female CHD8^+/- mice had social deficiencies (P<0.05). During the open field test, there was no significant difference in the total distance moved by male and female WT and CHD8^+/- mice. However, the amount of time spent in the central region was considerably lower in CHD8^+/- mice compared to the WT mice (P<0.01). Furthermore, the light-dark transition test revealed that both male and female CHD^8+/- mice spent considerably less time investigating the white box compared to the WT mice (P<0.05). Nevertheless, there were no notable alterations found in self-grooming, nesting, spontaneous alternation of Y-maze, and food burial experiments. In addition, Western blotting result  demonstrated a significant drop in doublecortin (DCX) expression (P<0.001), and immunofluorescent staining revealed a notable reduction in the number of DCX⁺ cells (P<0.01) in the hippocampus of CHD8^+/- mice.   Conclusion  CHD8^+/- mice exhibit social disorders and anxiety-like behaviors, with a decrease in the number of newly generated neurons in the hippocampus and neurogenesis disorders.