电针智三针调节酪氨酸激酶受体的配体通路改善血管性痴呆的作用机制

李霞; 朱世杰; 唐中生; 罗亚非; 范瑞娟; 谢高宇; 寇云芳; 陆莹

doi:10.16098/j.issn.0529-1356.2023.06.010

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解剖学报 ›› 2023, Vol. 54 ›› Issue (6) : 689-694. DOI: 10.16098/j.issn.0529-1356.2023.06.010

神经生物学

电针智三针调节酪氨酸激酶受体的配体通路改善血管性痴呆的作用机制

李霞¹ 朱世杰²唐中生² 罗亚非^2*范瑞娟² 谢高宇² 寇云芳² 陆莹²

作者信息 +

Mechanism of electroacupuncture regulating the ligand pathway of tyrosine kinase receptor in improving vascular dementia

LI Xia¹ ZHU Shi-jie² TANG Zhong-sheng² LUO Ya-fei^2* FAN Rui-juan² XIE Gao-yu² KOU Yun-fang² LU Ying²

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文章历史 +

摘要

目的观察电针智三针调节促红细胞生成素产生肝细胞受体B2/促红细胞生成素产生肝细胞配体B2/大丝裂原活化蛋白激酸1（EphB2/EphrinB2/BMK1）信号通路改善血管性痴呆大鼠神经损伤的潜在机制。方法 SD雄性成年大鼠80只，随机分为假手术组、模型组、非穴位电针组、尼莫地平组和电针智三针组，改良Pulsinelli四血管阻断法制作血管性痴呆大鼠模型，分组治疗后进行各组大鼠水迷宫实验，HE染色、Nissl染色和透射电子显微镜（TEM）观察海马CA1区病理变化，免疫组织化学法检测大鼠海马CA1区EphB2和BMK1表达，免疫印迹法检测大鼠海马CA1区EphB2和BMK1蛋白的表达情况。结果相比模型组，给予电针和尼莫地平治疗的血管性痴呆大鼠逃避潜伏期下降（P<0.05），而穿越平台的次数明显提高（P<0.05）；HE染色、Nissl染色和TEM结果显示，与模型组比较，电针智三针组、尼莫地平组大鼠的海马CA1区神经元排列较整齐，细胞及细胞器形态结构较完整；免疫组织化学法和免疫印迹法检测显示，与假手术组比较，模型组大鼠海马CA1区EphB2和BMK1的表达差异明显下降，差异有统计学意义（P<0.05）；与模型组比较，电针智三针组大鼠海马CA1区EphB2和BMK1的表达明显增加（P<0.05），非穴位电针组大鼠海马CA1区EphB2和BMK1的表达差异无统计学意义（P＞0.05），与尼莫地平组相比，电针智三针组大鼠海马CA1区EphB2和BMK1的表达明显增加（P<0.05）。结论电针智三针可能通过增加海马CA1区EphB2和BMK1的表达，改善血管性痴呆大鼠海马神经元的损伤，从而达到改善血管性痴呆大鼠的学习记忆。

Abstract

Objective To observe the potential mechanism of electroacupuncture regulating the erythropoietin-producing hepatocellular receptor B2/erythropoietin-producing hepatocellular receptor-interacting B2/big mitogen-activated protein kinase 1(EphB2/EphrinB2/BMK1) signaling pathway to improve neural damage in vascular dementia rats. Methods Eighty SD male adult rats were randomly divided into a sham surgery group, a model group, a non acupoint electroacupuncture group, a nimodipine group, and an electroacupuncture three needle group. The vascular dementia rat model was made by the modified Pulsinelli four vessel occlusion method. After grouping, the rats in each group were subjected to water maze test, HE staining, Nissl staining, and transmission electron microscopy(TEM) to observe the pathological changes in the hippocampal CA1 area, and the expression of EphB2 and BMK1 in the hippocampal CA1 area was detected by immunohistochemistry; Detection of EphB2 and BMK1 protein expression in rat hippocampal CA1 region was detected by Western blotting. Results Compared with the model group, the escape latency of vascular dementia rats treated with electroacupuncture and nimodipine decreased (P<0.05), while the number of crossing platforms significantly increased (P<0.05); The result of HE staining, Nissl staining and TEM showed that compared with the model group, the neurons in hippocampal CA1 area of rats in the EA Zhisanzhen group and nimodipine group were arranged orderly, and the morphology and structure of cells and organelle were complete; Immunohistochemical method and Western blotting showed that the expression of EphB2 and BMK1 in the CA1 region of hippocampus in the model group was significantly lower than that in the sham surgery group (P<0.05); Compared with the model group, the expression of EphB2 and BMK1 in the hippocampal CA1 region of rats in the electroacupuncture Zhisanzhen group significantly increased (P<0.05), while the expression of EphB2 and BMK1 in the hippocampal CA1 region of rats in the non acupoint electroacupuncture group was not statistically significant (P>0.05). Compared with the nimodipine group, the expression of EphB2 and BMK1 in the hippocampal CA1 region of rats in the electroacupuncture Zhisanzhen group significantly increased (P<0.05). Conclusion Electroacupuncture may improve the damage of hippocampal neurons in vascular dementia rats by increasing the expression of EphB2 and BMK1 in the CA1 region of the hippocampus, thereby improving the learning and memory of vascular dementia rats.

导出引用

李霞朱世杰唐中生罗亚非范瑞娟谢高宇寇云芳陆莹. 电针智三针调节酪氨酸激酶受体的配体通路改善血管性痴呆的作用机制[J]. 解剖学报. 2023, 54(6): 689-694 https://doi.org/10.16098/j.issn.0529-1356.2023.06.010

LI Xia ZHU Shi-jie TANG Zhong-sheng LUO Ya-fei FAN Rui-juan XIE Gao-yu KOU Yun-fang LU Ying. Mechanism of electroacupuncture regulating the ligand pathway of tyrosine kinase receptor in improving vascular dementia[J]. Acta Anatomica Sinica. 2023, 54(6): 689-694 https://doi.org/10.16098/j.issn.0529-1356.2023.06.010

中图分类号： R322

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