采用紫BL与蓝B2R对组织切片染色的观察

邵金辉 冯桂花

解剖学报 ›› 2020, Vol. 51 ›› Issue (1) : 132-134.

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解剖学报 ›› 2020, Vol. 51 ›› Issue (1) : 132-134. DOI: 10.16098/j.issn.0529-1356.2020.01.023
技术方法

采用紫BL与蓝B2R对组织切片染色的观察

  • 邵金辉* 冯桂花
作者信息 +

Observation of tissue slice staining with purple BL and blue B2R

  • SHAO Jin-hui* FENG Gui-hua
Author information +
文章历史 +

摘要

目的 寻找制备简单、效果良好、来源丰富的组织切片染色剂。 方法 应用27种直接服装染料对小鼠肝脏组织切片进行染色效果观察,发现其中的紫BL和蓝B2R染色能够清晰显示中央静脉和肝细胞核等结构,再用紫BL和蓝B2R对心脏、肾脏和睾丸组织切片进行染色效果观察。 结果 紫BL和蓝B2R用蒸馏水溶解后即可使用,配制简单,在5 min内完成。它们对心脏、肾脏和睾丸组织切片染色后,心肌横纹、肾小囊腔、生精细胞等结构清晰可辨,与HE染色结果类似。 结论 紫BL与蓝B2R比苏木素制备简单,同时它们的组织切片染色效果良好,来源丰富。因此,可能部分替代苏木素。

Abstract

 Objective To find out much more simply prepared, effective and abundant tissue slice dye.  Methods Totally 27 kinds of directly used dress dye were tested to make mouse liver slice stained. Among them purple BL and blue B2R made hepatic structure including central vein and hepatic nucleus shown clearly. Then, the heart, kidney and testis slices of mouse were stained by purple BL and blue B2R.  Results The solution preparation of Purple BL and blue B2R was just dissolved in distilled water in five minutes, so they were very much simple and timesaving.After the heart, kidney and testis slices of mouse were stained by purple BL and blue B2R,the stained tissue structure including cardiac muscle cross striation,kidney capsule cavity and spermatogenic cells is clear and similar to tissue stained by HE.  Conclusion The effective dress dye purple BL and blue B2R are much more simply prepared and abundant than hematoxylin. So, it can be partly replaced by purple BL and blue B2R.

关键词

服装染料 / / / / 睾丸 / 组织切片 / 染色 / 小鼠

Key words

Dress dye / Liver / Heart / Kidney / Testis / Tissue slice / Staining / Mouse

引用本文

导出引用
邵金辉 冯桂花. 采用紫BL与蓝B2R对组织切片染色的观察[J]. 解剖学报. 2020, 51(1): 132-134 https://doi.org/10.16098/j.issn.0529-1356.2020.01.023
SHAO Jin-hui FENG Gui-hua. Observation of tissue slice staining with purple BL and blue B2R[J]. Acta Anatomica Sinica. 2020, 51(1): 132-134 https://doi.org/10.16098/j.issn.0529-1356.2020.01.023
中图分类号: R329.3   

参考文献

[1] Li JCh, Zeng YSh. Histology and Embryology[M]. 9th eds. Beijing: People’s Health Publishing House, 2018:1-292.(in Chinese)
李继承, 曾园山. 组织学与胚胎学[M]. 第9版. 北京:人民卫生出版社,2018:1-292.
[2] Tang JM, Li JCh. Textbook of Histology and Embryology[M]. Beijing: Peking University Medical Press,2011:1-528. (in Chinese)
唐军民,李继承.组织学与胚胎学[M].北京:北京大学医学出版社,2011:1-528.
[3] Ovalle WK, Nahirney PC. Netter’s Essential Histology[M]. 2ed eds. London: Elsevier Health Sciences,2013:1-536.
[4] Bolton Hall AN, Joseph B, Brelsfoard JM, et al. Repeated closed head injury in mice results in sustained motor and memory deficits and chronic cellular changes[J].PLoS One,2016,11(7):e0159442.
[5] Ridley A. Silver staining of nerve endings in human digital glabrous skin[J]. J Anat,1969,104(Pt1):41-48. 
[6] Larson K, Ho HH, Anumolu PL, et al. Hematoxylin and eosin tissue stain in Mohs micrographic surgery: a review[J]. Dermatol Surg, 2011, 37(8):1089-1099. 
[7] Li Y, Cao L, Li J, et al. Influence of microgravity-induced intervertebral disc degeneration of rats on expression levels of p53/p16 and proinflammatory factors[J].Exp Ther Med, 2019,17(2):1367-1373.
[8] Gupta SK, Behera K, Pradhan CR, et al. Studies of the macroscopic and microscopic morphology (hippocampus) of brain in Vencobb broiler[J]. Vet World, 2016, 9(5):507-511.
[9] Cai WQ. Experimental Technique of Modern Practical Cellular and Molecular Biology[M]. Beijing: People’s Military Medical Publisher, 2003:66-76.(in Chinese)
蔡文琴.现代实用细胞与分子生物学实验技术[M].北京:人民军医出版社,2003:66-76.
[10]Sravya T, Rao GV, Kumari MG, et al. Evaluation of biosafe alternatives as xylene substitutes in hematoxylin and eosin staining procedure: A comparative pilot study[J].J Oral Maxillofac Pathol, 2018, 22(1):148. 
[11]Yee JY, Limenta LM, Rogers K, et al. Ensuring good quality RNA for quantitative real-time PCR isolated from renal proximal tubular cells using laser capture microdissection[J].BMC Res Notes,2014,7:62.

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