羽扇豆醇增强对沉默ST3GalⅢ基因的MDA-MB-231细胞侵袭转移的抑制作用

王明

doi:10.16098/j.issn.0529-1356.2020.01.005

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解剖学报 ›› 2020, Vol. 51 ›› Issue (1) : 26-31. DOI: 10.16098/j.issn.0529-1356.2020.01.005

肿瘤生物学

羽扇豆醇增强对沉默ST3GalⅢ基因的MDA-MB-231细胞侵袭转移的抑制作用

王明崔红霞刘吉成*

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Enhancing effect of Lupeol on migration and invasion abilities of ST3GalⅢ-silenced MDA-MB-231 cells#br# #br#

WANG Ming CUI Hong-xia LIU Ji-cheng*

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摘要

目的探索羽扇豆醇（Lupeol）增强对沉默ST3GalⅢ基因的人乳腺癌MDA-MB-231细胞侵袭转移的抑制作用。方法体外培养沉默ST3GalⅢ基因的MDA-MB-231细胞。采用细胞黏附实验，Transwell侵袭实验，细胞划痕实验检测羽扇豆醇对ST3GalⅢ沉默的MDA-MB-231细胞侵袭转移能力的作用。Western blotting检测各组细胞转移相关基质金属蛋白酶（MMP）-2、9和磷脂酰肌醇-3激酶/蛋白激酶B/核因子κB（PI3K/Akt/NF-κB）信号通路蛋白的表达情况。结果 ST3GalⅢ基因沉默及低浓度（5 μmol/L）羽扇豆醇对MDA-MB-231细胞的增殖及凋亡无明显影响。羽扇豆醇对ST3GalⅢ沉默的MDA-MB-231细胞黏附、迁移和侵袭有明显的抑制作用，且相关蛋白MMP-2、-9和PI3K/Akt/NF-κB信号通路蛋白表达均下调。结论羽扇豆醇体外能增强对沉默ST3GalⅢ基因的人乳腺癌MDA-MB-231细胞侵袭和转移的抑制作用，其机制可能与抑制MMP-2、-9的蛋白表达及影响了PI3K/Akt/NF-κB信号通路有关。

Abstract

Objective In this study, we expored the enhancing effect of Lupeol on migration and invasion abilities of ST3GalⅢ-silenced MDA-MB-231 cells. Methods Human breast cancer cell line ST3GalⅢ -silenced MDA-MB-231 was cultured in vitro. The cell adhesion, Transwell and woud healing test were utilized to test the effect of Lupeol on ST3GalⅢ-silenced MDA-MB-231 cells. The expression of matrix metalloproteinase(MMP)-2, -9, phosphatidylinositol 3-kinase/protein kinase B/nuclear factor κB(PI3K/Akt/NF-κB) signaling pathway were examined by Western blotting. Results No significant influence of the decreased expression of ST3GalⅢ and Lupeol（5μmol/L）on proliferation and apoptosis of MDA-MB-231 cells were found. Lupeol inhibited the migration and invasion of ST3GalⅢ-silenced MDA-MB-231 cells in vitro(P<0.05). Furthermore, the expression of NF-κB p65, p-Akt, Akt, p85, MMP-2 and MMP-9 levels were significantly down-regulated. Conclusion These observations suggest that Lupeol may inhibit the abilities of migration and invasion of ST3GalⅢ-silenced MDA-MB-231 cells in vitro by inhibiting the protein expression of MMP-2, -9 and effect the signaling pathway of PI3K/Akt/NF-κB.

关键词

Lupeol / ST3GalⅢ / Breast cancer cell / Migration / Invasion / Western blotting / Human

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王明崔红霞刘吉成. 羽扇豆醇增强对沉默ST3GalⅢ基因的MDA-MB-231细胞侵袭转移的抑制作用[J]. 解剖学报. 2020, 51(1): 26-31 https://doi.org/10.16098/j.issn.0529-1356.2020.01.005

WANG Ming CUI Hong-xia LIU Ji-cheng. Enhancing effect of Lupeol on migration and invasion abilities of ST3GalⅢ-silenced MDA-MB-231 cells#br# #br#[J]. Acta Anatomica Sinica. 2020, 51(1): 26-31 https://doi.org/10.16098/j.issn.0529-1356.2020.01.005

中图分类号： R730.2

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