白细胞介素6对脂多糖诱导所致类子痫前期大鼠有核红细胞的影响

刘丽平; 徐慧; 朱梦妮

doi:10.16098/j.issn.052-1356.2023.06.015

PDF(7886 KB)

解剖学报 ›› 2023, Vol. 54 ›› Issue (6) : 722-729. DOI: 10.16098/j.issn.052-1356.2023.06.015

组织学胚胎学发育生物学

白细胞介素6对脂多糖诱导所致类子痫前期大鼠有核红细胞的影响

刘丽平¹ 朱梦妮¹ 徐慧^2*

作者信息 +

Effect of interleukin-6 on nucleated erythrocytes in lipopolysaccharide induced preeclampsia rats

LIU Li-ping¹ ZHU Meng-ni¹ XU Hui^2*

Author information +

文章历史 +

摘要

目的探讨白细胞介素(IL)-6对脂多糖(LPS)诱导类子痫前期大鼠有核红细胞的影响。方法采用ELISA和免疫组织化学法检测正常妊娠、轻和重度子痫前期患者外周血与胎盘中IL-6的表达；采用流式细胞术和免疫荧光法检测母体有核红细胞比例。SD妊娠大鼠随机分为3组：对照组、LPS组和LPS+anti-IL-6组，检测外周血和胎盘中IL-6、有核红细胞比例、JAK2/MEK和PI3K/Akt信号基因表达。结果子痫前期患者的IL-6明显高于正常妊娠者。相比于对照组，LPS组外周血和胎盘中IL-6、有核红细胞比例和JAK2、P85、Akt、P65、IL1B mRNA表达明显增加，胎鼠体重明显降低；相比于LPS组，LPS+anti-IL-6组外周血和胎盘中IL-6表达、有核红细胞比例、JAK2、P85、Akt、P65和IL-1B mRNA表达明显减少。结论子痫前期患者外周血和胎盘IL-6上调伴随外周血有核红细胞增多，在体中和IL-6可能通过下调有核红细胞JAK2/PI3K/Akt/NF-κB信号介导的IL-1B表达从而保护类子痫前期大鼠。

Abstract

Objective To explore the effect of interleukin (IL)-6 on nucleated erythrocytes in lipopolysaccharide (LPS)-induced preeclampsia rats. Methods ELISA and immunohistochemistry were used to detect the IL-6 in peripheral blood and placenta of preeclampsia and normal pregnancy; Flow cytometry and immunofluorescence were used to detect the maternal nucleated erythrocytes. Pregnant SD rats were randomly divided into 3 groups: the control, LPS and LPS +anti-IL-6 group; IL-6, the proportion of nucleated erythrocytes, JAK2/MEK and PI3K/Akt signal-related genes were detected. Results The IL-6 of preeclampsia was higher than that of normal patients. Compared with the Control group, IL-6, the proportion of nucleated erythrocytes and JAK2, P85, Akt, P65, IL-1B mRNA of LPS group increased, the fetal weight decreased; Compared with the LPS group, IL-6, the proportion of nucleated erythrocytes and JAK2, P85, Akt, P65 and IL-1B mRNA of the LPS+anti-IL-6 group decreased. Conclusion The up-regulation of IL-6 of preeclampsia patients is accompanied by increased nucleated erythrocytes in peripheral blood. Neutralizing IL-6 in vivo may down-regulate JAK2/PI3K/Akt/NF-κB-signal-mediated IL-1B to protect preeclampsia rats.

导出引用

刘丽平朱梦妮徐慧.

白细胞介素6对脂多糖诱导所致类子痫前期大鼠有核红细胞的影响

[J]. 解剖学报. 2023, 54(6): 722-729 https://doi.org/10.16098/j.issn.052-1356.2023.06.015

LIU Li-ping ZHU Meng-ni XU Hui.

Effect of interleukin-6 on nucleated erythrocytes in lipopolysaccharide induced preeclampsia rats

[J]. Acta Anatomica Sinica. 2023, 54(6): 722-729 https://doi.org/10.16098/j.issn.052-1356.2023.06.015

中图分类号： R285.5

参考文献

［1］Ives CW, Sinkey R, Rajapreyar I, et al. Preeclampsia-pathophysiology and clinical presentations: JACC state-of-the-art review［J］. J Am Coll Cardiol，2020, 76(14): 1690-1702.

［2］Yang Z, Zhang WY. Interpretation of the "Guidelines for the Diagnosis and Treatment of Pregnancy Induced Hypertension (2020)" ［J］. Chinese Journal of Obstetrics and Gynecology, 2020, 55(6): 425-432 (in Chinese)

杨孜，张为远. 《妊娠期高血压疾病诊治指南(2020)》解读［J］. 中华妇产科杂志, 2020, 55(6): 425-432.

［3］Cui L, Takada H, Takimoto T, et al. Immunoregulatory function of neonatal nucleated red blood cells in humans［J］. Immunobiology, 2016, 221(8): 853-861.

［4］Hu G, Guan R, Li L. Nucleated red blood cell count in maternal peripheral blood and hypertensive disorders in pregnant women［J］. Am J Med Sci, 2016, 351(2): 140-146.

［5］Aggarwal R, Jain A K, Mittal P, et al. Association of pro-and anti-inflammatory cytokines in preeclampsia［J］. J Clin Lab Anal, 2019, 33(4): e22834.

［6］Wang G J, Yang Z, Huai J, et al. Pravastatin alleviates oxidative stress and decreases placental trophoblastic cell apoptosis through IL-6/STAT3 signaling pathway in preeclampsia rats［J］. Eur Rev Med Pharmacol Sci, 2020, 24(24): 12955-12962.

［7］Ding H, Dai Y, Lei Y, et al. Upregulation of CD81 in trophoblasts induces an imbalance of Treg/Th17 cells by promoting IL-6 expression in preeclampsia［J］. Cell Mol Immunol, 2019, 16(1): 302-312.

［8］Ferber A, Minior V K, Bornstein E, et al. Fetal "nonreassuring status" is associated with elevation of nucleated red blood cell counts and interleukin-6［J］. Am J Obstet Gynecol, 2005, 192(5): 1427-1429.

［9］Hu G, Guan R, Li L. Nucleated red blood cell count in maternal peripheral blood and hypertensive disorders in pregnant women［J］. Am J Med Sci, 2016, 351(2): 140-146.

［10］Hebbar S, Misha M, Rai L. Significance of maternal and cord blood nucleated red blood cell count in pregnancies complicated by preeclampsia［J］. J Pregnancy, 2014, 2014: 496416.

［11］Pitman H, Innes BA, Robson SC, et al. Altered expression of interleukin-6, interleukin-8 and their receptors in decidua of women with sporadic miscarriage［J］. Hum Reprod, 2013, 28(8): 2075-2086.

［12］Saggini A, Chimenti S, Chiricozzi A. IL-6 as a druggable target in psoriasis: focus on pustular variants［J］. J Immunol Res, 2014, 2014: 964069.

［13］Ibrahim MH, Moustafa AN, Saedii A, et al. Cord blood erythropoietin and cord blood nucleated red blood cells for prediction of adverse neonatal outcome associated with maternal obesity in term pregnancy: prospective cohort study［J］. J Matern Fetal Neonatal Med, 2017,30(18):2237-2242.

［14］Pitman H, Innes BA, Robson SC, et al. Altered expression of interleukin-6, interleukin-8 and their receptors in decidua of women with sporadic miscarriage［J］. Hum Reprod, 2013,28(8):2075-2086.

［15］Hebbar S, Misha M, Rai L. Significance of maternal and cord blood nucleated red blood cell count in pregnancies complicated by preeclampsia［J］. J Pregnancy, 2014,2014:496416.

［16］Wang H, Wang Z, Wang L, et al. IL-6 promotes collagen-induced arthritis by activating the NLRP3 inflammasome through the cathepsin B/S100A9-mediated pathway［J］. Int Immunopharmacol, 2020,88:106985.