Research on fetal brain tissue holds immense significance for neuroscience and disease mechanisms, as its developmental processes form the cornerstone for understanding brain development and its abnormalities. The formulation of a standardized operational protocol for China's fetal brain tissue bank is urgently needed, as it can fill the gaps in the domestic relevant standardized system, support basic research and clinical exploration, and assist in addressing challenges related to ethics and sample quality. The establishment of this protocol aims to ensure operational consistency, minimize experimental deviations, provide reliable samples for disease research, and enhance international competitiveness. By integrating the practical experience of multiple domestic brain banks, this standardized operational protocol summarizes the objectives of fetal brain tissue bank collection, proposes the legal basis and ethical considerations for fetal collection, outlines the content of clinical data collection and the procedures for fetal brain tissue sampling, briefly describes pathological diagnosis norms, and details the preservation and management of information related to fetal brain tissue. Additionally, it innovatively introduces postmortem fetal brain magnetic resonance imaging (ex vivo MRI) technology. The protocol aims to establish unified and standardized criteria, strengthen the collection, processing, and sharing of samples, and promote the advancement of brain science research.