Expression of Bmi-1 in different developmental stage of esophageal epithelium tissues

ZHOU Wen-Bin; LIU Yang; SHI Su-Yun; CHEN Xiao-Xue; PEI Xue-Lian; MU Xiao-Ling

Acta Anatomica Sinica ›› 2016, Vol. 47 ›› Issue (1) : 95-101.

histology,embryology and developmental biology

Expression of Bmi-1 in different developmental stage of esophageal epithelium tissues

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Abstract

Objective To observe the Bmi-1 protein expression in esophageal epithelial tissues ,and to explore the relationship between the expression pattern of Bmi-1 and the development of esophageal epithelial tissues. Methods Immunohistochemistry was used to measure the expression of Bmi-1 in esophageal epithelial tissues of 120 cases mouse in different development time,15 cases human embryos, 18 cases normal adult human and 17 cases human esophageal squamous cell carcinoma (ESCC), then the level of Bmi-1 protein expression in these samples were analyzed by image analysis software, and the statistical analyses were applied to test the relationship between the expression of Bmi-1 and each group. Results Bmi-1 was found to expression in every time of development, but the differences were present in the protein expression level (P＜0.05). Expression of Bmi-1 protein was higher at embryo stage than that after birth both in mice and human. Compared with esophageal tissue of normal adult human, Bmi-1 overexpressed both in human esophageal squamous cell carcinoma and human embryo(P＜0.01). Conclusions The expression of Bmi-1 closely was related with the development of esophageal epithelium.Furthermore,there were a potential significance to detection of the Bmi-1 expression for the research in the developmental biology of esophageal epithelium and the cytobiology of esophageal cancer.

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Bmi-1 / esophageal epithelium / development / Immunohistochemistry

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Expression of Bmi-1 in different developmental stage of esophageal epithelium tissues[J]. Acta Anatomica Sinica. 2016, 47(1): 95-101

References

[1]Haupt Y, Alexander W S, Barri G, et al.Novel zinc finger gene implicated as myc collaborator by retrovirally accelerated lymphomagenesis in E mu-myc transgenic mice [J]. Cell, 1991, 65(5):753-763.
[2]Park IK,Qian D,Kiel M, et al . Bmi-1 is required for maintenance of adult self-renewing haematopoietic stem cells [J].Nature 2003, 423:302-305.
[3]Leung C,Lingbeek M,Shakhova O,et al.Bmi1 is essential for cerebellar development and is overexpressed in human medulloblastomas [J].Nature 2003,428:337-341.
[4]郑翔宇,朱杰,王艺芳等. Bmi-1-siRNA对肺腺癌A549细胞体内外增殖能力的影响[J]. 中国癌症杂志,2013,07:505-511.
[5]Yoshikawa R, Tsujimura T, Tao L, et al. Theoncoprotein and stem cell renewal factor BMI1 associates with poorclinical outcome in oesophageal cancer patients undergoing preoper-ative chemoradiotherapy [J].BMC Cancer 2012;12:461.
[6]Alkema MJ,Wiegant J,Raap AK,et al. Charaeterization and ehromosomal loealizationof the human Proto-oneogene Bmi-1[J].Hum Mol Genet 1993,2(10):1597-1603.
[7]Park IK,Qian D,Kiel M, et al . Bmi-1 is required for maintenance of adult self-renewing haematopoietic stem cells [J].Nature 2003, 423:302-305.
[8]Lessard J, Sauvageau G et al. Bmi-1 determines the proliferative capacity of normal and leukaemic stem cell [J].Nature 2003, 423:255-260.
[9]Molofsky AV, Pardal R, Iwashita T, et al. Bmi-1 dependence distinguishes neural stem cell self-renewal from progenitor proliferation [J].Nature, 2003, 425: 962-967.
[10] Chatoo W, Abdouh M, Duparc RH, et al. Bmil distinguishes immature retinal progenitor/stem cells from the main progenitor cell population and is required for normal retinal development [J].Stem Cells 2010, 28:1412-1423.
[11]Cordisco S, Maurelli R, Bondanza S, Stefanini M, et al. Bmi-1 reduction plays a key role in physiological and premature aging of primary human keratinocytes [J].J Invest Dermatol. 2010, 130(4):1048-62.
[12]Sangiorgi E, Capecchi MR,et al. Bmi-l is expressed in vivo in intestinal stem cells [J].Nature Genetics 2008, 40:915-920.
[13]Siddique, H.R. M. Saleem, et al. Role of Bmi-1, a stem cell factor, in cancer recurrence and chemoresistance: preclinical and clinical evidences [J].Stem Cells, 2012, 30(3):372-378.
[14] Bonnie C,?Santhoshi B,?XiaYL,et al. Clinicopathologic characteristics of high expression of Bmi-1 in esophageal adenocarcinoma and squamous cell carcinoma [J].BMC Gastroenterology ,2012 ,12:146.
[15] He XT,Cao XF,Ji L,et a1.Association between Bmi-l and clinicopathological status of esophageal squamous cell carcinoma [J].World J Gastroenterol,2009,15(19):2389-2394.
[16] Lv J , Cao XF ,Ji L, Zhu B et a1.Association of β-catenin, Wnt1, Smad4, Hoxa9, and Bmi-1 with the prognosis of esophageal squamous cell carcinoma [J].Med Oncol 2012, 29:151–160.
[17].Kim T. H，Lee H. M.Lee S. H，et al.Expression and distribution patterns of the stem cell marker, nestin, and the stem cell renewal factor, BMI-1, in normal human nasal mucosa and nasal polyps [J].Acta Oto-laryngologica, 2009. 129(9): 996-1001.
[18]Pan Q, A.M. Nicholson, Barr H, et al.Identification of Lineage-Uncommitted, Long-Lived, Label-Retaining Cells in Healthy Human Esophagus and Stomach, and in Metaplastic Esophagus[J]. Gastroenterology 144 (4): 761-770.
[19] He S,Iwashita T,Buchstaller J,et al. Bmi-1 over-expression in neural stem/progenitor cells increases proliferation and neurogenesis in culture but has little effect on these functions in vivo [J]. Developmental Biology, 2009. 328(2):257-272.
[20]张世庆. 精原干细胞自增殖分子机制研究[D].中国人民解放军军事医学科学院,2007.
[21]阮艳. 过表达癌基因Bmi-1促进鼠源胚胎干细胞自我更新[D].第三军医大学,2009.
[22]Hwang CC, Nieh S, Lai CH,et al.A retrospective review of the prognostic value of ALDH- 1, Bmi-1 and Nanog stem cell markers in esophageal squamous cell carcinoma [J ].PLoS ONE 2014 9(8): e105676.