Established the 3D anatomy interaction system of zebrafish (Danio rerio)

LIN Jin-Xing; ZHANG Lin-Li; HU Jian-Hua; GAO Cheng; FENG Li-Ping; WEI Xiao-Feng; CHEN Wei; CHEN Qiu-Sheng

Acta Anatomica Sinica ›› 2016, Vol. 47 ›› Issue (1) : 139-144.

technology and methodology

Established the 3D anatomy interaction system of zebrafish (Danio rerio)

Author information +

History +

Abstract

Objective The aim of this paper was to make a software of three-dimension (3D) anatomical interaction system in zebrafish (Danio rerio). Methods The structure of zebrafish was observed by light microscopy and Micro-CT, and the images of its morphological structure were collected. The pictures of serial section (HE-stained) were corrected by Photoshop. The 3D model was established by the software of Autodesk 3ds Max. Results The results showed the zebrafish skeletal 3D imaging and serial section images (male: 144, female: 179). We realized the 3D reconstruction of the vital organs of zebrafish, and established the 3D anatomy interaction system zebrafish (V 1.0), which obtained the computer software copyright registration certificate (Certificate Number: soft word board the Di_0989966). Conclusion The zebrafish 3D anatomy interaction system software afforded the three-dimensional morphological basis for the study of zebrafish, and was helpful to histomorphology recognization for researchers.

Key words

zebrafish / Micro-CT / serial section / picture correction / three-dimension (3D) reconstruction

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

Established the 3D anatomy interaction system of zebrafish (Danio rerio)[J]. Acta Anatomica Sinica. 2016, 47(1): 139-144

References

[1] Experimental animals seminars - infectious disease and common diseases [EB/OL]. [2015-06-15]. http://www.calas.org.cn/html/zxbg/tzgg/20150415/2273.html
实验动物疾病研讨会—传染性及常见疾病[EB/OL]. http://www.calas.org.cn/html/zxbg/tzgg/20150415/2273.html, 20150615.
[2] Lin JX, Zhang LL, Yao YL, et al. Micro- and ultrs-structure of the intestine in zebrafish (Danio rerio)[J]. Anim Husb &Vet Med, 2013, 45(3): 60-66.
林金杏, 章琳俐, 姚一琳, 等. 斑马鱼肠道显微和超微结构的研究[J].畜牧与兽医, 2013, 45(3): 60-66.
[3] Chu XH, Pu JY, Bao HJ, et al. Observation of Micro- and ultra-structures of the liver in zebrafish (Brachydanio rerio)[J]. Lab Anim & Comp Med, 2010, 30(1): 17-23.
初晓红, 濮俊毅, 包惠君, 等. 斑马鱼正常肝脏显微形态和超微结构的初步观察[J].实验动物与比较医学, 2010, 30(1): 17-23.
[4] Yao Y, Lin J, Chu X, et al. Fine structure, enzyme histochemistry and immunohistochemistry of liver in zebrafish[J]. Anat Rec, 2012, 295(4): 567-576.
[5] Chen W, Zhang LL, Yang P, et al. Fine morphology and structure of telecephlon in zebrafish (Danio rerio)[J]. J Fisher Chin, 2015, 39(3): 371-380.
陈微, 章琳俐, 杨平, 等. 斑马鱼端脑的微细形态与结构[J]. 水产学报, 2015, 39(3): 371-380.
[6] Zhang L, Yang P, Liu Y, et al. Pre-spermiogenic initiation of flagellar growth and correlative ultrastructural observations on nuage, nuclear and mitochondrial developmental morphology in the zebrafish Danio rerio[J]. Micron, 2014, 66: 1-8.
[7] Wyatt C, Bartoszek EM, Yaksi E. Methods for studying the zebrafish brain: past, present and future[J]. Eur J Neurosci, 2015, doi: 10.1111/ejn.12932. [Epub ahead of print]
[8] Sun YH. A brief introduction for the national resource center for zebrafish [J]. Hereditas, 2013, 35(4): 549-550.
孙永华. 国家斑马鱼资源中心简介[J]. 遗传, 2013, 35(4): 549-550.
[9] Shi W, Fang Z, Li L, et al. Using zebrafish as the model organism to understand organ regeneration[J]. Sci China Life Sci, 2015, 58(4): 343-51.
[10] Li HY, Pan LY, Sun YH. Development and maintenance of zebrafish resources, and the China zebrafish resource center [J]. Acta Lab Anim Sei Si, 2014, 22(6): 93-98,105.
李阔宇, 潘鲁湲, 孙永华. 斑马鱼资源的开发保藏与国家斑马鱼资源中心[J]. 中国实验动物学报, 2014, 22(6): 93-98,105.
[11] He JL, Liu J, Wang TQ, et al. Research of zebrafish quality standardization [J]. Acta Lab Anim Sei Si, 2014, 22(6): 99-102.
何嘉玲, 刘静, 王天奇, 等. 斑马鱼的质量标准化[J]. 中国实验动物学报, 2014, 22(6): 99-102.
[12] Hawkins P, Dennison N, Goodman G, et al. Guidance on the severity classification of scientific procedures involving fish: report of a working group appointed by the Norwegian Consensus-Platform for the Replacement, Reduction and Refinement of animal experiments (Norecopa) [J]. Lab Anim, 2011, 45(4): 219-224.
[13] Liu C, Cheng SJ, Luo YN, et al. Introduction and comparative anaIysis of the revised directive 2010/63/EU for the protection of laboratory animals[J]. Chin J Comp Med, 2012, 22(5): 72-76.
刘超, 程树军, 罗苑妮, 等. 欧盟保护实验动物新指令2010/63/EU介绍及比较分析[J].中国比较医学杂志, 2012, 22(5): 72-76.
[14] Ji DF, Sun LY, Song JM, et al. Three dimensional reconstruction of hippocampus structure in rat brain based on histological serial sections[J]. Fudan Univ J Med Sci, 2013,40(2):217-221.
季达峰, 孙李颖, 宋佳敏, 等. 组织学切片法在三维重建大鼠海马结构中的应用价值[J].复旦学报(医学版), 2013,40(2):217-221.
[15] Dun J, Cao L, Liu F, et al. Three-dimensional reconstruction of rat small intestine[J]. Acta Anat Si, 2011, 42(3): 421-424.
杜鹃,曹雷,刘锋, 等. 大鼠小肠壁连续切片的三维重建[J]. 解剖学报, 2011, 42(3): 421-424.
[16] Lu ZJ, Yao ZT, Yao GY, et al. Three-dimensional reconstruction of mini-pig skin microvasculars in meridlans[J]. Acta Anat Si, 2011, 42(3): 425-428.
吕钊君, 摇张涛, 摇郭洋, 等. 小型猪经脉区皮肤微血管的三维重建[J]. 解剖学报, 2011, 42(3): 425-428.
[17] Schulz-Mirbach T, He? M, Metscher BD. Sensory epithelia of the fish inner ear in 3D: studied with high-resolution contrast enhanced microCT[J]. Front Zool, 2013, 10(1):1-11.
[18] Zhu FD, Shi J, Song E, et al. Digital 3D anatomical modeling of the mandible with full teeth[J]. Acta Anat Si, 2014, 42(4): 536-539.
朱赴东, 石珏, 宋恩, 等. 数字化全牙列下颌骨三维解剖建模[J]. 解剖学报, 2014, 42(4): 536-539.
[19] Wang J, Liu Y, Li X, et al. Three-dimensional visualization models of maxillofacial region based on CT and MRI images using a personal computer[J]. Chin J Tiss Engin Res, 2014, 18(36): 5788-5792.
王娇，刘洋，李旭, 等. PC机上基于CT、MRI图像构建的颌面部三维数字模型[J].中国组织工程研究, 2014, 18(36): 5788-5792.
[20] Bourassa D, Gleber SC, Vogt S, et al. 3D imaging of transition metals in the zebrafish embryo by X-ray fluorescence microtomography[J]. Metallomics. 2014, 6(9): 1648-1655.
[21] Chu XH, Pu JY, Yao YL, et al. Research on Bile System in Zebrafish (Brachydanio rerio)[J]. Lab Anim & Comp Med, 2010, 30(3): 167-173.
初晓红, 濮俊毅, 姚一琳, 等. 斑马鱼肝脏胆管系统的研究[J]. 实验动物与比较医学, 2010, 30(3): 167-173.
[22] Zhang LL, Yao YL, Chu XH, et al. Structure and composition of intestinal mucosal barrier in zebrafish (Brachydanio rerio)[J]. Lab Anim & Comp Med, 2013, 33(2):112-116.
章琳俐, 姚一琳, 初晓红, 等. 斑马鱼肠黏膜屏障的结构与组成[J]. 实验动物与比较医学, 2013, 33(2):112-116.
[23] Zhang L, Yang P, Ullah S, et al. Fine Structure of Zebrafish (Danio rerio) Spermatozoa[J]. Pak Vet J, 2014, 34(4): 518-521.
[24] Lin JX, Wei XF, Chu XH, et al. Establishment the virtual system of zebrafish liver three-dimensional structure[J]. Lab Anim & Comp Med, 2012, 32(5): 390-395.
林金杏, 魏晓锋, 初晓红, 等. 斑马鱼肝脏三维结构虚拟系统的建立[J]. 实验动物与比较医学, 2012, 32(5): 390-395.