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Expression of oligodendrocyte transcription factor and neurological cadherin in the developing spinal cord of the human embryo
ZHANG Yong ZHANG Ju-shi CHEN Jian-er LIU Xue-hong*
Acta Anatomica Sinica ›› 2015, Vol. 46 ›› Issue (5) : 655-659.
Expression of oligodendrocyte transcription factor and neurological cadherin in the developing spinal cord of the human embryo
Objective To investigate distribution of the oligodendrocyte transcription factor (Olig) and neurological cadherin（N-cadherin） in the developing human embryonic spinal cord and their clinical significance. Methods Immunohistochemistry was uesd to detect the expression and distribution of Olig and N-cadherin proteins in the spinal cord of 17 human embryos at the second, third or fourth month gestation. One-Way ANOVA and LSD-t test were employed to compare positive expression number and intensity values of Olig and N-cadherin proteins in spinal cord tissues detected in the 3 fetal periods. Results At the second to fourth gestation, the positive expressions of Olig and N-cadherin were seen in the human embryonic spinal cord. With the increase of gestational age, the intensity values of N-cadherin positive cells were gradually increased in the spinal cord, and positive expression intensity values were 117.77±6.72，129.63±9.44 and 133.35±8.15(P<0.01), respectively. Olig positive expression intensity values showed an up and then down trend were 69.98±24.59，85.10±9.19 and 71.62±11.81(P<0.05), respectively. The number of Olig positive cells in the spinal cord were gradually increased and were 9.38±7.80，15.55±7.30，25.59±14.54 (P<0.01), respectively. The results of Olig and N-cadherin showed a significant difference in the spinal cord. Conclusion At the second to fourth gestation, the positive expressions of N-cadherin and Olig suggest that they are closely related to the nerve and glial cells differentiation and development in the spinal cord tissues during the human embryonic growth and development.
Embryo /
Spinal cord /
Oligodendrocyte transcription factor /
Neurological cadherin /
Immunohistochemistry /
Human
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