Objective This study was designed to investigate the BHD gene expression location in human lung cancer cells and its effect on the invasion ability of lung cancer cells. Methods The BHD vector and the small interfer RNA targeting BHD was constructed and transfected into the cell line A549. The expression of BHD was examined by immunofluorescence. Transwell test was used to detect the invasion ability of A549 cells. Results After transfected BHD vector into A549 cells, immunofluorescence showed the expression of Folliculin located in cellular plasma. The invasion ability of A549 cells decreased after transfected with BHD vector, and the invasion ability of A549 cell increased after transfected with BHD siRNA. Conclusion In the lung cancer cell line A549, Folliculin is expressed in the cytoplasm and BHD gene is a metastasis suppressor gene.