Objective To study the expression pattern of fibroblast growth factor receptor 3(FGFR3) in mouse embryonal limb bud development. Methods Affymetrix mouse 430 2.0 cDNA was used with microarray technique to examine the 34 000 genes. Histology staining observation was performed at the same time. Results FGFR3 expression was enhanced obviously in mouse E12.5d embryonal limb bud. It continued to increase and peaked in mouse E13.5d embryonal limb bud. Furthermore, the expression down-regulated gradually from E14.5d in mouse embryonal limb bud. Conclusion The expression of FGFR3 has close correlation with chondrogenesis of mouse embryonal limb bud. FGFR3 may play an important role in chondrogenesis.