Objective To investigate the localization and the morphological changes of manchette during mouse spermiogenesis. Methods Immunofluorescence staining with FITC and costaining with DAPI were used to demonstrate the cellular localization of the manchette at different stages during mouse spermiogenesis. The structural changes of the manchette were observed during the maturing of the spermatid. Results Immunofluorescence staining showed that manchette existed exactly around the nuclei of the spermatids. Manchette began to form, when the shape of the nucleus changed from spherical to slightly elongated. While the nucleus of the spermatids condensed and elongated at later stages, manchette moved gradually to the caudal position of the spermatids. At last, the manchette diminished as the spermatids became mature. During mouse spermiogenesis，manchette underwent a transition from a cap-like to a tubular configuration. ConclusionThe formation and diminishment of the manchette is in step with the condensation and elongation of the nucleus of the spermatid. Both the structural and positional changes of the manchette coincide with the changes of the nucleus. These results imply that manchette might play an important role in mouse spermiogenesis.