Objective To study the effect of hydrogen sulfide (HSUB>2/SUB>S) on intracellular calcium concentration and proliferation in hepatic stellate cells (HSC) and the possible mechanism of HSUB>2/SUB>S. Methods HSC-T6, an activated rat HSC, was plated in 35-mm culture dish at a density of 1×10SUP>5/SUP>/ml, and rotuinely incubated in DMEM media for 24 hours. The intracellular CaSUP>2+/SUP> loaded by Flu-3/AM was measured by laser scanning confocal microscope. The dynamic changes of intracellular CaSUP>2+/SUP> fluorescence intensity(［CaSUP>2+/SUP>］i ) stimulated by NaSH, a HSUB>2/SUB>S donor, were measured. The HSC proliferations were measured by MTT at different HSUB>2/SUB>S concentrations. Results Low concentration of HSUB>2/SUB>S(100μmol/L) decreased significantly HSC-T6 ［CaSUP>2+/SUP>］i (16.20±3.56 vs 14.12±3.76,EM>P/EM><0.05). The increased HSC-T6 proliferations were also observed in low concentration stimulation of HSUB>2/SUB>S(50-100μmol/L). The decreased ［CaSUP>2+/SUP>］i was inhibited by glibenclamide, a KSUB>ATP/SUB> channel antagonist， significantly when administered with HSUB>2/SUB>S of higher concentrations. However, high concentration of HSUB>2/SUB>S(1mmol/L) increased significantly HSC-T6 ［CaSUP>2+/SUP>］i，P<0.05, with no significant changes in proliferation. Conclusion Th