Objective To study the expression of growth-arrest-specific protein 7 (Gas7) in the hippocampus of rats after chronic multiple-stress. Methods thirty-six Wistar rats were randomly divided into two groups: the chronic multiple stressed group and the control group. Rats in the stressed group were administered with four kinds of stressors including vertical rotation，sleeping deprivation，restraint(6hours/day) and illumination irregularly and alternately for 6 weeks. Then the expression of Gas7 protein in the hippocampus of rats was assayed by immunocytochemical method and Western blotting technique respectively and apoptotic cells were observed. Results The expression of Gas7 was extensively found in the hippocampus of both the control group and the experimental group mainly in the endochylema and processes of the neurons in the CA1 area. Positive staining, average absorbance in the CA1 and dentate gyrus of the stressed rats were higher than that in the control group (P<0.05). There was no difference in the expressions in the CA3 areas in the two groups (P>0.05). Caspase-3 positive cells were also obserued in the stressed rats. Western blotting detection indicated that the expression of Gas7 in the stressed rats was significantly higher than that of the control group(P<0.05).Conclusion Gas7 may participate in protecting neurons in the stess and promote the neurogenesis an development of neurons.