Objective To evaluate the relationship between the RUNX3 gene, cell turnover(apoptosis and proliferation) and survival in gastric cancer patients. Methods RUNX3 was examined using immunohistochemical staining. Cell turnover was analyzed by flow cytometry using standard techniques. Survival was evaluated by KaplanMeier survival curves. BR> Results The positiveexpression rate of RUNX3 in tumor tissue from 60 patients with gastric cancer was 46.7%. RUNX3 positiveexpression was correlated with lymph node metastasis and distant metastasis(EM>P/EM><0.05). Normal distribution was found by frequency distribution of AI/PI in gastric carcinoma cells. Tumor cells exhibited a wide range of AI/PI expression from 0.05 to 0.46Cell turnover was also correlated with lymph node metastasis and distant metastasis (P<0.05).It was determined that the expression of RUNX3 and cellturnover rates were associated (EM>r/EM>=0.39，EM>P/EM>0.05). Using KaplanMeier survival curves and theEM> Log-rank/EM> test, there was also a correlation between RUNX3 and survival(EM>P/EM><0.05). Conclusion The RUNX3 gene may be related with tumorigenesis and tumor progression by affecting cell turnover, The detection of RUNX3 may be an impor