Objective To explore the capability of chicken embryonic primordial germ cells (EPGCs) to differentiate into adipocytes and neuronlike cells, and to compare the inductive effect of a variety of specific chemical inductive media and their coordination with variant inductive programmer. Methods The chicken EPGCs at stage 19 and 28 were cultured and subcultured to the 4th passage, and then were differentiated into adipocytes and neuronlike cells respectively. EPGCs differentiated cells were then identified by oil redO. EPGCs were similarly induced into neuronlike cells in the medium containing RA, BHA, IBMX and DMSO; the differentiated cells were identified by toluidine blue stain and specific immunohistochemistry. Results About 74%-91% oil redO positive EPGCs were induced into adipocytes after 14-21 days. Programmer 2 showed the best result（P<0.01）. The differentiation rates of EPGCs from stage 19 and 28 were 89% and 91% respectively. The cooperative inductive effect was significantly higher than the single inductive effect of desamethason, insulin and IBMX. However, in the same induction media, the oil redO stained cells showed no significant difference between stage 19 and stage 28（P>0.05）. The specific PPARγ gene cDNA had been proliferatied from induced adipocytes. And 75%85% toluidine blue positive EPGCs were induced into neuronlike cells after 3-7 days. The average differentiation rates were 82% and 83% for inductive media RA and IBMX, which were significantly higher(P<0.01）than the cooperative inductive effect of RA,BHA,DMSO and IBMX. There was no signifi