Objective To compare the advantages and disadvantages of three methods showing the blood supply of peripheral nerve, and provide morphological basis for vascularized nerve grafts. Methods The blood supply of brachial plexus and its main branches were observed on fresh cephalothoraxupper limb specimen or adult cadavers, using the perfusion method by red latex, Kato method by ink, and contrast radiography method by gelatinlead oxide respectively. Results The blood supply of brachial plexus and its main branches were displayed by all the three methods, but each method had its own characteristics: the perfusion method by latex showed the extrinsic blood supply of the peripheral nerve; Kato method by ink showed the intrinsic blood supply of the peripheral nerve; contrast radiography method by gelatinlead oxide showed the extrinsic and intrinsic blood supply of the peripheral nerve.Conclusion The perfusion method by red latex, Kato method by ink and contrast radiography method by gelatinlead oxide can be applied respectively to the studies of extrinsic, intrinsic and integral topography of blood s