Objective To observe the expression of EGFR，TGF-β，AQP-2 and Bax during development of renal tubules in chick embryo，and explore the role and regulating significance of them. Methods The expression of EGFR，TGF-β，AQP-2 and Bax were observed by immunohistochemistry combined with stereological methods in 36 chicks kidney at 10th-46th incubation stages. The expression intensity of them was analyzed by IPP software. Results The epithelial cells of proximal tubules in chick embryo appeared EGFR immunoreactivity at 26th-46th stages；At 26th-37th stages the epithelial cells of proximal tubules and distal tubules of mesonephros appeared TGF-β immunoreactivity，the epithelial cells of proximal tubules and distal tubules of metanephros appeared TGF-β immunoreactivity at 33rd-40th stages，at 40th-46th stages the epithelial cells of proximal tubules of metanephros appeared TGF-β immunoreactivity but the epithelial cells of distal tubules did not appeare TGF-β immunoreactivity；The epithelial cells of collecting tubules in chick embryo appeared AQP-2 immunoreactivity at 26th-46th stages；The epithelial cells of proximal tubules and distal tubules of mesonephros appeared Bax immunoreactivity at 26th-37th stages，the epithelial cells of proximal tubules of metanephros appeared Bax immunoreactivity at 33rd-46th stages. Images showed the expressions of EGFR，TGF-β（βSUB>1/SUB>，β SUB>2/SUB>，βSUB>3/SUB>）and Bax first displayed up then down，but expression of AQP-2 always displayed up during develop