Objective To observe the ultrastructure changes on von Ebner’s gland of type 2 diabetes rats. Methods Eighteen Wistar rats were randomly divided into control group and experiment group. The diabetes rats model were induced by intraperitoneal injection streptozotocin (STZ) after highfat food.The von Ebner’s gland ultrastructure was observed under transmission electron microscope （TEM） after 8 weeks. Results Compared with the control group, in diabetes rats acinar cell nucleus appeared nucleolemma introcession and karyopycnosis; mitochondria exhibited vacuole degeneration and crista breaking; rough endoplasmic reticula expanded and showed degranulation.Conclusion A sereies of pathologic changes in von Ebner’s gland of diabetes rats can be induced by diabetes, which provides morphological evidences fo