Objective To evaluate the existence of basic fibroblast growth factor(bFGF) and transforming growth factorbeta(TGF-β)in the sclera in different fetal ages by realtime polymerase chain reaction (PCR). Methods Forty human fetal eyes ranging in age from 12 to 20 pregnant weeks were carefully extracted. The sclera was carefully dissected. The expressions of bFGFand TGF-β were determined by realtime PCR, and the results were analyzed comparatively. Results bFGF was expressed in the highest amount at the 12th to 14th pregnant week and decreased thereafter, while the expression of TGF-β lowered at the 12th week and increased gradually.Conclusion bFGF and TGF-β express in the sclera of the human fetal