Objective To detect the expressions of annexin 5 mRNA and Bax mRNA in the rat testis after the submandibular gland was removed. Methods On days 14, 28 and 42 after the operation, the expression levels of annexin 5 and Bax mRNA in the rat testis were examined by fluorogenic quantitative RT-PCR. Results Fluorogenic quantitative RT-PCR showed that there was a 38.5% and 55.3% increase in the expression of annexin 5 mRNA and a 70.6% and 80.5% significant increase in the expression of Bax mRNA (P<0.05, P<0.01) in the rat testis on days 14 and 28 respectively. There was no significant change in the expressions of annexin 5 and Bax mRNA on day 42 compared with the control groups.Conclusion The removal of submandibular gland can significantly increase the expressions of annexin 5 and Bax mRNA in the rat testis on days 14 and 28. The expressions of annexin 5 and Bax mRNA goes back to the normal level on day 42