Objective To examine the expression and distribution of Aquaporin 1 （AQP1） in the seminal vesicle and prostate gland of male mice during postnatal development. Methods The RT-PCR for AQP1 mRNA, Western blotting for protein, and immunohistochemistry for distribution in the seminal vesicle and prostate gland on postnatal day 15, day 35 and day 70 were carried out. Results The results of RT-PCR and Western blotting showed that the level of AQP1 mRNA and protein were significantly increased in the seminal vesicle and prostate gland on postnatal day 70 (P<0.05). The positive signals of AQP1 protein were localized immunohistochemically in the smooth muscle cells of seminal vesicle and the basement membrane of glandular epithelium and the mesenchyme cells of prostate gland on postnatal day 15 and day 35, but it was hardly detected in the epithelium of seminal vesicle. Thereafter, AQP1 protein was intensively expressed in the epithelium of seminal vesicle and prostate gland on postnatal day 70.Conclusion The different expressions and distributions of AQP1 mRNA and protein in the seminal vesicle and prostate gland of mice in different ages may be related to in the development of male accessory glands.