Objective To study the effect of Nogo-A gene silence on dopamine release in PC12 cells. Methods The small hairpin RNA(shRNA) eukaryotic expression vector targeting Nogo-A gene was constructed and transfected into cultured PC12 cells by lipofecamine2000. The inhibiting effect was detected by semiquantitative reverse transcription(PCR) and Western blotting analysis. The dopamine release was detected by HPLC(high performance liquid chromatography) after transfection. Results The Nogo-A gene expression was inhibited and the dopamine release decresed significantly 48 hours after transfection.Conclusion The reasults suggest that Nogo-A be involved in the mechanism of dopamine realese in PC12 cells.