Objective To explore the possible function and significance of pancreatic polypeptide during the healing process of experimental gastric ulcer in rat. Methods The immunohistochemical PAP method,morphometry and image analysis were applied to study the changes of the morphology, numerical density on area(N\-A) and mean grey degree of islet PP cells during the healing process of experimental gastric ulcer in rats. Results Compared with normal control group(NCG) and saline control group(SCG), the N\-A of PP cells markedly decreased, and the mean grey degree markedly increased(P<0.05) on the 6th day after the experimental gastric ulcer group(EUG). During the 10th and the 28th day after the process of experimental gastric ulcer, the N\-A of PP-positive cell increased,and the mean grey degree decreased(P<0.05),especially on the 10th day.The results of morphological observation and image analysis in pancreas islet PP cells were basically the same during the healing process of experimental gastric ulcer. Conclusion During the healing process of experimental gastric ulcer,the N\-A and mean grey degree of PP-positive cell both changed.It indicated that PP cells played a role in the modulating process of experimental gastric ulcer.