Objective To determinted whether GM1 had a protective effect on injury induced by serumdeprivation and the possible mechanism in PC12 cells. Methods The viability of PC12 cells was quantified by MTT after serumdeprivation. The number of apoptotic cells and necrotic cells were determined by Hoechst 33258/PI staining. And the change of PKC protein expression on PC12 cells’ membrane and cytosols was detected by Western blotting. Results 1The viability of PC12 cells decreased after serumdeprivation and the serum-deprivation for 24 hours was chosen as an injury model in this research. Most of the PC12 cells presented apoptosis 24 hours after serumdeprivation. In addition, the PC12 cells’ cytosols PKC protein decreased, while the PC12 cells’ membrane PKC protein increased significantly, and this result suggested PKC’s translocation to membrane and its activation. 2The viability of PC12 cells preincubated with GM1 in high concentrations(10,1,01μmol/L) increased significantly and GM1 protected PC12 cells from apoptosis after serumdeprived injury. GM1 reduced the damage of serumdeprivation on PC12 cells and inhibited PKC protein translocation after injury.3The repair fun