Objective To investigate the distribution and role of hepcidin on ferroportin1 and divalent metal transporter 1(DMT1) expression in the mouse brain. Methods The expression of hepcidin in the mouse brain was detected by RT_PCR. The role of hepcidin on ferroportin1 and DMT1 expression was studied with cerebral ventricle injection and immunohistochemistry methods. Results There was hepcidin mRNA expression in the brain, and the level of hepcidin mRNA varied in different brain areas. Abundant expression of hepcidin existed in the choroid plexus.Conclusions The positive staining of DMT1, ferroportin1 was changed remarkably after the injection of hepcidin into cerebral ventricle of the brain; the change of DMT1 and ferroportin1 staining was regionally distinct.