Objective To explore whether ganoderma spores could help the neural epithelial cell of embryonic neural tube stagnated by retinoic acid in G\-0/G\-1 phase reenter the cell cycle, keep on proliferating and differentiating, and could reduce the occurrence of neural tube defects (NTDs). Methods When E7.75d, mice of the control group and the ganoderma spores group were given intragastrically onetime retinoic acid all-trans. Then the ganoderma spores solution was given intragastrically to the ganoderma spores group. At E10.5d, The embryos of the two groups were taken out and the ratio of NTDs was counted respectively Immunofluorescence histochemistry and flow cytometry were applied to examine the expression of nestin, and DNA quantity staining and RT-PCR to detect the mRNA transcriptions of Cdk2 and Cdk4. Results The radio of NTDs in the ganoderma spores group was lower than that in the control group remarkably, but the level of nestin was higher. With the neural tube cell cycles compared the cell radio of G\-0/G\-1 phase in the control group was higher than that in the ganoderma spores group, while the ratio in the S phase in the control group, it was lower than that of the normal embryos in the ganoderma spores group. The neural epithelial cell of embryonic neural tube in the ganoderma spores group could transcript Cdk4 mRNA normally, with a low transcription rate in the control group.Conclusion Ganoderma spores can reduce the occurrence of embryonic NTDs induced by retinoic acid in pregnant mice.