Objective To construct a highly efficient eukaryotic expression vector carrying human peroxisome proliferatoractivated receptor δ(hPPARδ) gene in order to provide an ideal molecular platform for screening natural ligands and functional study of hPPARδ. Methods hPPARδ gene, cloned from total RNA of HepG2 cells by RT-PCR, was ligated with pIRES2EGFP plasmid which was excised by BamHI and SalI double endonucleases. The recombinant plasmid was transfected into 293 cells. Realtime quantitative PCR and immunocytochemistry assays were used to analyze the expression levels of hPPARδ in the transfected 293 cells. Results hPPARδ gene sequence contained in the recombinant plasmid phPPARδIRES2EGFP was verified correct by enzyme digestion as well as sequence analysis. After being transfected into 293 cells, high efficient expression of hPPARδ gene contained in phPPARδIRES2EGFP plasmid was found to display a highly efficient expression detected both at mRNA and protein levels by r