Objective To investigate the distribution of protein kinase C gamma (PKCγ) in medulla oblongata and white matter of the spinal cord in adult rats. Methods Immunohistochemical staining technique(ABC method) was used to demonstrate the localization of PKCγ immunoreactivities. Results PKCγ immunoreactivities were located in the pyramid, decussation of pyramid and the ventral part of posterior funiculus in the cervical, thoracic, lumbar and sacral segments of the spinal cord. In addition, PKCγ immunoreactivities were also expressed in the neurons at dorsal horn of medullary and different segments of the spinal cord. Conclusion The distribution of PKCγ in medulla oblongata and white matter of the spinal cord in adult rats are correspondent with the position and course of the rat corticospinal tract. The widespread expression of PKCγ in corticospinal tract in adult rats indicate