Objective To compare the ratios of neuron and glia differentiated from the embryonic human neural stem cells(hNSCs) at different times in vitro. Methods The cells from hippocampus of aborted human embryo tissue(8-12 weeks after conception) were mechanically dissociated and cultured with DMEM/F 12 medium containing h-EGF, h-bFGF, h-LIF. The NSCs were induced to differentiate by 1%FBS and identified by Musashil, β-Ⅲ Tubulin, GFAP and Galc immuno-cytochemical staining at different times. Results The cell spheres were Musashil positive at the 12th hour after differentiation.On the 2nd day after differentiation,a few cells exhibited β-Ⅲ Tubulin and GFAP immunoreactivities,but no Galc positive cells.The number of β-Ⅲ Tubulin immuno-positive cells reached the highest on the 7th day and the percent was 48.2%.The number of GFAP immuno-positive cells increased from the 2nd to the 23rd day and the positive ratio reached 65.3%.A few Galc immuno-positive cells were detected with time passing. Conclusion 1% FBS may promote human NSCs to differentiate into three cell types of central nervous