P>Objective To investigate the expressions of enhancer of zeste(EZH2) in carcinoma of esophagus and its potential significance. Methods The expression and distribution of EZH2 were determined on the esophageal squamous carcinoma, adenocarcinoma by using immunohistochemistry. We then detected EZH2 protein expression in the esophageal squamous carcinoma, paracarcinomatous and normal tissues by Western blotting. Results The immunohistochemical staining showed EZH2 protein was highly expressed in esophageal squamous carcinoma and adenocarcinoma tissues. The positive staining was observed in the nuclear region. Normal tissues and corresponding paracarcinomatous tissues were stained weakly. Western blotting analysis showed that the strongest positive signals were detected in the esophageal squamous carcinoma (P<0.01). The positive signals were stronger in corresponding paracarcinomatous tissue than that in normal tissues (P<0.05). The expression of EZH2 protein in invasive and metastatic carcinomas had strikingly increased, compared with carcinomas without invasion and metastases, which had significant differences in the statistics (P<0.05). The expression of EZH2 in well differentation squamous carcinoma was significantly lower than that in medium or poor differentiation respectively. However, the expression in well differentation adenocarcinoma was significantly higher than that in medium or poor differentiation. Conclusion EZH2 protein was highly expressed in esophageal carcinoma. The expression of EZH2 protein was clearly associated with invasion, metastases and tissue types of esophageal carcinoma./P>