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Differentiation of cardiomyocyte-like cells from human umbilical cord-derived mesenchymal stem cells induced by valsartan
HE Wan-li LI Yong-hai YANG Jun-zheng LIN Jun-tang GUO Zhi-kun XU Zhen-ping*
Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (6) : 841-846.
Differentiation of cardiomyocyte-like cells from human umbilical cord-derived mesenchymal stem cells induced by valsartan
Objective To explore the feasibility of differentiating the human umbilical cord-derived msenchymal stem cells (hUCMSCs) to myocardiocyte-like cells after induction with valsartan. Methods The hUCMSCs were induced by valsartan and cultured for 1 week, 2 weeks and 3 weeks respectively. The antibodies against cardiac troponin T (cTnT）and recombinant GATA binding protein 4 (GATA4）were used to identify the cells which were induced to differentiate by immunocytochemistry and immunofluorescence. The mRNA expressions of cardiac troponin I(cTnI), myocardial-related transcription factor and myocardial development-related genes were detected by RT-PCR to identify the feasibility of differentiating hUCMSCs and inducing capacity of valsartan. Results The morphology of normal hUCMSCs showed uniform fibrous and whirlpool-like shape. They became significantly smaller and short spindle shape after valsartan induction. Immunochemical and immunofluorescence results showed that the expression of cTnT and GATA4 protein in the induced group was positive and seldom in the control group. RT-PCR results showed that the mRNA expression of cardiac-related transcription factor Tbx5, GATA4 and Nkx2.5 was spontaneously and continuously in the hUCMSCs. They increased after being induced by valsartan 1 week and then declined. The mRNA expression of cTnI was detected at 3 weeks, but the atrial natriuretic polypeptide（ANP）and β-myosin heavy chain (β-MHC) were not detected. Conclusion The hUCMSCs have capacity to be induced to differentiate into cardiomyocyte-like cells by valsartan.
Valsartan /
Inducation /
Differentiation /
Cardiomyocyte-like cell /
Immunohistochemistry /
Immunofluorescence /
Human umbilical cord-derived msenchymal stem cell
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