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Gastrodin inhibits lipopolysaccharide-induced autophagy in human umbilical vein endothelial cells
XIE Ya-nan YANG Ping WEN Fei SUN Lin CHEN Yuan-li* LU Di*
Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (6) : 824-829.
Gastrodin inhibits lipopolysaccharide-induced autophagy in human umbilical vein endothelial cells
Objective To investigate the regulation mechanism of gastrodin (Gas) on atutophagy of human umbilical vein endothelial cells（HUVECs）stimulated with lipopolysaccharide (LPS). Methods Gas at 5, 10, 20 and 50μmol/L was added 1h before 1mg/L LPS stimulation, after 12h incubation. The protein and mRNA expression of Beclin-1, ATG5 and LC3 were detected by Western blottingting and RT-PCR respectively. The autophagosome was observed under fluorescent microscope by monodansylcadaverine (MDC) staining. Cell viability rate was measured by MTT analysis. Results Autophagy was induced in HUVECs by LPS-stimulation. The protein and mRNA expression of Beclin-1, ATG5 and LC3 were significantly increased,which was in a time-dependent manner. Compared with LPS-stimulated group, pertreatment with Gas inhibited the expression of autophagy in dose-dependent manner and the cell viability was effectively increased. Conclusion Autophagy was induced in HUVECs by LPS-stimulation. Gas may increase the viability rate of HUVECs through inhibiting the expression of autophagy.
Gastrodin / Lipopolysaccharide / Autophagy / Monodansylcadaverine / Western blotting / RT-PCR / Human umbilical vein endothelial cell
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