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Influence of annexin A7 knockdown on expression of Bcl-2 and Bax of HepG2 cells
WANG Xiao-jie LIANG Xiu-jun LI Xin*
Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (6) : 809-813.
Influence of annexin A7 knockdown on expression of Bcl-2 and Bax of HepG2 cells
Objective To investigate the influence of annexin A7 knockdown on apoptosis and expression of Bcl-2 and Bax of HepG2 cells. Methods HepG2 cells cultured in six-well plates were divided into 3 groups, siRNA interference group, negative control group and blank control group. The siRNA interference group was transfected with the siRNA which target annexin A7, the negative control group was transfected with negative siRNA, in the blank control group only transfection reagent was added. Western blotting was used to identify the siRNA targeted to annexin A7 can highly surpress annexin A7 expression in 48h after transfection. Flow cytometry was used to detect the apoptotic rate. Immunohistochemistry and Western blotting were applied to observe the expression of Bcl-2 and Bax protein; RT-PCR was performed to detect the expression of Bcl-2 and Bax mRNA. Results The apoptotic rate increased and the expression of Bcl-2 decreased significantly compared with the negative control group and the blank control group(P<0.05), while the expression of Bax protein and mRNA had no significant difference when compared with the negative control group and the blank control group(P>0.05). Conclusion Annexin A7 knockdown can promote apoptosis of HepG2 cells, reduce the ratio of Bcl-2 and Bax.
Annexin A7 / HepG2 cell / Bcl-2 / Bax / Flow cytometry / Human
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