Welcome to visit Acta Anatomica Sinica! Today is
Chinese
Effects of curcumin on proliferation and adipogenic differentiation of porcine bone marrow mesenchymal stem cells
ZHANG Qing-mei LI Fang-zheng JIANG Zhong-ling SUN Dong-xing LI Dong-jian SONG Xue-xiong*
Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (6) : 793-799.
Effects of curcumin on proliferation and adipogenic differentiation of porcine bone marrow mesenchymal stem cells
Objective To investigate the effects and mechanisms of curcumin on proliferation and adipogenic differentiation of porcine bone marrow mesenchymal stem cells (BMSCs). Methods The porcine BMSCs were cultured and differentiated with cell culture and adipegenic differentiation medium containing different concentrations of curcumin, the effects of curcumin on proliferation of porcine BMSCs were detected by MTT, the effects of curcumin on adipogenic differentiation were analyzed by morphology and oil red O, and the expressions of peroxisome proliferator-activated receptor γ(PPARγ2) mRNA and lipoprotein lipase(LPL) mRNA were detected by Real-time PCR. Results When the porcine BMSCs were cultured with curcumin, 1μmol/L curcumin at the third days of culture and 5, 10μmol/L curcumin at the fifth days of culture significantly promoted cell proliferation (P<0.05), but curcumin concentration higher than 15μmol/L inhibited cell proliferation (P<0.05). When the porcine BMSCs were differentiated with curcumin, the different concentrations of curcumin significantly inhibited adipogenic differentiation (P<0.05). The inhibition rate of 0.5μmol/L curcumin was 28.3% and 20μmol/L curcumin was 71.24%. When the cells were treated with 20μmol/L curcumin and differentiated in 5, 10 and 15 days, the inhibition rates of expressions of PPARγ2 and LPL mRNA were 19.11%, 49.52%, 76.76% and 37.58%, 49.32%, 74.70% respectively.
Conclusion The proper concentrations of curcumin were able to promote the proliferation and inhibit adipogenic differentiation of porcine BMSCs, suggesting that curcumin may play an important role in the regulation of adipocyte differentiation.
Bone mesenchymal stem cell / Curcumin / Proliferation / Adipogenic differentiation / Real-time PCR / Porcine
［1］Bray GA, Bellanger T. Epidemiology,trends, and morbidities of obesity and the metabolic syndrome［J］. Endoerine, 2006, 29(1): 109-117.
［2］Prins JB, O’Rahilly S. Regulation of adipose cell number in man ［J］. Clin Sci, 1997, 92(1):3-11.
［3］Reis JP, Loria CM, Lewis CE, et al. Association between duration of overall and abdominal obesity beginning in young adulthood and coronary artery calcification in middle age［J］. JAMA, 2013, 310(3): 280-288.
［4］Logue J, Murray HM, Welsh P, et al. Obesity is associated withfatal coronary heart disease independently of traditional risk factors and deprivation［J］. Heart, 2011, 97(7): 564-568.
［5］Gupta SC, Patchva S, Aggarwal BB. Therapeutic roles of curcumin: lessons learned from clinical trialsP［J］. AAPS J, 2013, 15(1): 195-218.
［6］Hatcher H, Planalp R, Cho J, et al. Curcumin: from ancient medicine to current clinical trials［J］. Cell Mol Life Sci, 2008, 65(11):1631-1652
［7］Su J, Xu ZP. Research progress of pharmacological effects of curcumin［J］. Jiangxi Journal of Traditional Chinese Medicine, 2010, 41(8): 528. (in Chinese)
苏菁, 徐宗佩. 姜黄素药理作用研究进展［J］. 江西中医药, 2010, 41(8): 528.
［8］Wang BH, Zhang WH, Zhang XF, et al. Research progress on pharmacology and dosage form of curcumin［J］. Chinese Archives of Traditional Chinese Medicine, 2013, 31(5): 1102-1105. (in Chinese)
王彬辉, 章文红, 张晓芬, 等.姜黄素的药理及剂型研究进展［J］. 中华中医药学刊, 2013, 31(5): 1102-1105.
［9]Liu HY, Wang HY, Ye S, et al. Research progress of curcumin pharmacological action and mechanism［J］. China Journal of Modern Medicine, 2012, 22(6): 48-51. (in Chinese)刘红艳, 王海燕, 叶松, 等. 姜黄素药理作用及其机制研究进展［J］. 中国现代医学杂志, 2012, 22(6): 48-51.
［10］Kim CY, Le TT, Chen C, et al. Curcumin inhibits adipocyte differentiation through modulation of mitotic clonal expansion［J］. J Nutr Biochem, 2011, 22(10): 910-920.
［11］Asma Ejaz A, Wu D, Kwan P, et al. Curcumin inhibits adipogenesis in 3T3-L1 adipocytes and angiogenesis and obesity in C57/BL mice［J］. J Nutr, 2009, 139(5): 919-925.
［12］Qian H, Xu WR, Zhang XR, et al. Basic biologial characteristics, isolation, and purification of mesenchymal stem cells drived from embryonic bone marrow［J］. Acta Anatomica Sinica, 2004，35(4): 440-442. (in Chinese)
钱晖, 许文荣, 张锡然, 等. 胚胎骨髓间质干细胞分离纯化及基本生物学特性［J］. 解剖学报, 2004，35(4): 440-442.
［13］Zhang HW, Bai L, Li HX, et al. Swine-the ideal animal model of obesity and diabetes［J］. Progress in Natural Science, 2008, 18(5): 481-487. (in Chinese)
张浩卫, 白亮, 李惠侠, 等. 猪研究肥胖和糖尿病的理想模式动物［J］. 自然科学进展, 2008, 18(5): 481-487.
［14］Kobayashi E, Hishikawa S, Teratani T, et al. The pig as a model for translational research: overview of porcine animal models at Jichi Medical University［J］. Transplant Res, 2012, 1(1):8.
［15］Wen Y, Sun ChH, Liu R. Effect of curcumin on the proliferation and differentiation of 3T3-L1 preadipocytes［J］. Chinese Journal of Clinical Rehabilitation, 2006,10(31): 117-119. (in Chinese)
闻颖, 孙长颢, 刘荣. 姜黄素对3T3-L1前脂肪细胞增殖分化的影响［J］. 中国临床康复, 2006,10(31): 117-119.
［16］Zheng J, Bai YN, Song ZhF, et al. Effect of curcumin on proliferation and differentiation of SW872 adipocytes［J］. Medical Recapitulate, 2013, 19(16) : 2991-2993. (in Chinese)
郑佳, 白宇娜, 宋振锋, 等. 姜黄素对SW872细胞增殖及分化的影响［J］. 医学综述, 2013, 19(16) : 2991-2993.
［17］Yang ChZh, Di L, Yu Y, et al. Effects of curcum in on insulin resistancein simple obesity rats［J］. Chinese General Practice, 2009, 12(7B): 1293-1295. (in Chinese)
杨成志, 狄灵, 于燕,等. 姜黄素对单纯性肥胖大鼠胰岛素抵抗的影响［J］. 中国全科医学, 2009, 12(7B): 1293-1295.
［18］Seo KI, Choi MS, Jung UJ, et al. Effect of curcumin supplementation on blood glucose, plasma insulin, and glucose homeostasis related enzyme activities in diabetic db/db mice［J］. Mol Nutr Food Res, 2008, 52(9): 995-1004.
［19］Gu Q, Cai Y, Huang C, et al. Curcumin increases rat mesenchymal stem cell osteoblast differentiation but inhibits adipocyte differentiation［J］. Pharmacogn Mag, 2012, 8(31): 202-208.
［20］Ahn J, Lee H, Kim S, et al. Curcumin-induced suppression of adipogenic differentiation is accompanied by activation of Wnt/β-catenin signaling［J］. Am J Physiol Cell Physiol, 2010, 298(6): 1510-1516.
［21］Zhao J, Sun X B, Ye F, et al. Suppression of fatty acid synthase, differentiation and lipid accumulation in adipocytes by curcumin［J］.Mol Cell Biochem, 2011, 351(1-2): 19-28.
/
| 〈 |
|
〉 |
