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Effect of angelica polysaccharide on hematopiesis in aging rats and its biological mechanism
ZHANG Yan-yan LI Jing JIA Dao-yong ZHANG Meng-si XU Chun-yan JING Peng-wei LI Cheng-peng WANG Lu WANG Shun-he WANG Ya-ping*
Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (6) : 785-792.
Effect of angelica polysaccharide on hematopiesis in aging rats and its biological mechanism
Objective To explore the effect and mechanism of angelica sinensis polysaccharide（ASP）on hematopiesis in aging rats and to provide theoretical and experimental evidences for effective natural medicine of antiaging or protecting hematopoietic function. Methods Male SD rats（n=40）aging from 6 to 8 weeks old were randomly divided into normal group(NG, n=10)，ASP normal group(ANG, n=10), aging model group(AMG, n=10) and ASP aging model group(AAMG, n=10). After 2 days of the treatment, the eyeball blood was collected for the peripheral blood routine test; The femur was taken to count the number of each femur bone marrow mononuclear cells (BMNCs). The proliferation of BMNCs was detected by CCK-8. The distribution of cell cycles and reactive oxygen species (ROS) levels were analyzed by flow cytometry(FCM). The capability of colony formation was examined by CFU-Mix cultivation. The ratio of the SA-β-Gal staining positive BMNCs was counted. The total antioxidant capacity of the cell was detected by enzymatic assay. The aging related proteins of P53 and P21 were detected by Western blotting. Results Compared with the aging model group, in the ASP aging model group,the decrease in the amounts of the peripheral blood RBC, PLT and WBC was evidently inhibited; the proliferation of the BMNCs was enhanced;the colony formation of CFU-Mix was markedly increased; the percentage of SA-β-Gal, the ratio of G1 stages and the product of ROS positive cells were significantly reduced; the ratio of S stages was markedly promoted; the total antioxidant capacity of the cell was significantly promoted; the expression of the aging related protein of P53 and P21 was evidently down-regulated.
Conclusion ASP can protect hematopoietic function from decline by antagonize D-galactose that induced oxidative stress, suggesting the mechanism of ASP protecting hematopiesis may regulate p53/p21 pathway.
Angelica polysaccharide / Aging model / Bone marrow mononuclear cell / Western blotting / Rat
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