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Cadmium damages the blood-testis barrier in rats and the protective effect of Astragaloside IV
NING Wei LIAO Xiao-gang* WANG Yi YAO Zhi-yong MAO Sheng-yan
Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (5) : 704-709.
Cadmium damages the blood-testis barrier in rats and the protective effect of Astragaloside IV
Objective To observe the effect of astragaloside IV (A) and SB203580 antagonize cadmium(Cd) toxicity on expression of associated protein and blood-testis barrier(BTB) in rats and to study the protective mechanism of A on it. Methods Totally 21 SD male rats were randomly divided into 7 groups, 3 rats per group：Cd ［intraperitoneally injected with 0.1%CdCl2，1mg/(kg·d)］，Cd+A ［at the above dose of CdCl2，at the same time with A，10mg/(kg·d)］，and Cd + SB203580 ［at the above dose of CdCl2，at the same time with SB203580，100μg/(kg·d)］， each of the above groups was further divided into continuous five and ten days treatment groups. The control group was intraperitoneally injected with equal dosage of normal saline. The testes were studied by light, electron microscopy, immunohistochemistry and Western blotting. Results In the control group，irregular and lightly stained nuclei of Sertoli cell(Sc) in seminiferous tubules were observed by HE staining. A continuous electron density line of tight junction（TJ）and normal ultrastructure of BTB were observed. After Cd treatment，the vesicular formation in the Sc was observed. The ultrastructural damage of Sc and TJ was observed. Compared with the corresponding time point of Cd group，these were weakened in morphology of testis and ultrastructure of TJ after Cd + A or Cd + SB203580 treatment. The positive products of zonula occludens-1（ZO-1） and claudin-11 were localized mainly in the base of the seminiferous tubule. After Cd treatment，the average absorbance (AA) of ZO-1 and Claudin-11 was decreased significantly compared with that of the control group（P＜0.05）. After Cd + A or Cd + SB203580 treatment，AAof ZO-1 and Claudin-11 were increased significantly compared with that of the Cd group（P＜0.05），though lower than that of the control group. The result of Western blotting showed that phosphorylation-p38MAPK in Cd group was increased significantly compared with that of the control group（P＜0.05）.After Cd + A or Cd + SB203580 treatment, it was decreased significantly compared with that of the Cd group（P＜0.05）. Conclusion Cd decreases ZO-1 and Claudin-11 expression and damages ultrastructure of TJ in BTB, as Ⅳ has protective effect on it, and is related to inhibiting activation of p38 MAPK pathway.
Cadmium / Astragaloside IV / Zonula occludens-1 / Claudin-11 / P-p38 MAPK / Immunohistochemistry / Western blotting / Rat
［1］He P, Lu Y, Liang Y, et al. Exposure assessment of dietary cadmium: findings from shanghainese over 40 years, China［J］. BMC Public Health,2013,13:590.
［2］Siu ER, Mruk DD, Porto CS, et al.Cadmium-induced testicular injury［J］.Toxicol Appl Pharmacol,2009,238(3):240-249.
［3］Lee J, Lim KT. Expression of TNF-α and IL-6 in HMC-1 cells treated with bisphenol A is attenuated by plant-originating glycoprotein (75 kDa) by blocking p38 MAPK［J］.Naunyn Schmiedebergs Arch Pharmacol,2010,382（1）:51-61.
［4］Mao ShY, Liao XG, Jiang R, et al. Protective function of astragaloside on cadmium-treated Sertoli cell in rat ［J］. Laser Journal, 2011,32(4):84-86.(in Chinese)
毛胜艳, 廖晓岗, 姜蓉, 等. 黄芪甲苷对镉致大鼠支持细胞损伤的保护作用［J］.激光杂志,2011,32（4）:84-86.
［5］Shi ZhH, Liao XG, Zou C, et al. Protective effect of astragalus mongholicus against cadmium toxicity on the expression of vimentin & e-cadherin and the ultrastructure of Sertoli cell in rats ［J］. Chinese Journal of Histochemistry and Cytochemistry, 2012,21(3):284-289.(in Chinese)
石之虎, 廖晓岗, 邹聪, 等. 黄芪对镉致大鼠睾丸支持细胞超微结构及波形蛋白、E-钙黏蛋白表达改变的保护作用［J］.中国组织化学与细胞化学杂志,2012,21（3）:284-289.
［6］Chang YX, Sun YG, Li J, et al.The experimental study of astragalus membranaceus on meridian tropsim: the distribution study of astragaloside IV in rat tissues［J］.J Chromatogr B Analyt Technol Biomed Life Sci,2012,911:71-75.
［7］Li X, Wang X, Han C, et al. Astragaloside IV suppresses collagen production of activated hepatic stellate cells via oxidative stress-mediated p38 MAPK pathway［J］.Free Radic Biol Med,2013,60:168-176.
［8］Pérez CV, Theas MS, Jacobo PV, et al. Dual role of immune cells in the testis: protective or pathogenic for germcells［J］? Spermatogenesis,2013,3(1):e23870.
［9］Erkanl ?entürk G, Ersoy Canilliolu Y, Umay C, et al. Distribution of zonula occludens-1 and occludin and alterations of testicular morphology after in utero radiation and postnatal hyperthermia in rats［J］.Int J Exp Pathol，2012，93(6):438-449.
［10］Lui WY, Lee WM, Cheng CY. Transforming growth factor-beta3 perturbs the inter-Sertoli tight junction permeability barrier in vitro possibly mediated via its effects on occludin, zonula occludens-1, and claudin-11［J］. Endocrinology,2001,142(5):1865-1877.
［11］Lydka M, Bilinska B, Cheng CY,et al. Tumor necrosis factor α-mediated restructuring of the Sertoli cell barrier in vitro involves matrix metalloprotease 9 (MMP9), membrane-bound intercellular adhesion molecule-1 (ICAM-1) and the actin cytoskeleton［J］.Spermatogenesis,2012,2(4):294-303.
［12］Xiao X, Cheng CY, Mruk DD. Intercellular adhesion molecule-2 is involved in apical ectoplasmic specialization dynamics during spermatogenesis in the rat［J］. J Endocrinol,2013,216(1):73-86.
［13］Wong CH, Mruk DD, Lui WY, et al. Regulation of blood-testis barrier dynamics: an in vivo study［J］. J Cell Sci.,2004,117（5）:783-798.
［14］Siu ER, Wong EW, Mruk DD, et al. An occludin-focal adhesion kinase protein complex at the blood-testis barrier: a study using the cadmium model［J］. Endocrinology,2009,150(7):3336-3344.
［15］Mazaud-Guittot S. Dissecting the phthalate-induced Sertoli cell injury: the fragile balance of proteases and their inhibitors［J］.Biol Reprod,2011,85(6):1091-1093.
［16］Qu YZ, Li M, Zhao YL, et al. Astragaloside IV attenuates cerebral ischemia-reperfusion-induced increase in permeability of the blood-brain barrier in rats［J］. Eur J Pharmacol,2009,606(1-3):137-141.
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