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Effect of lipopolysaccharide on the iron metabolism in macrophages
WANG Li JIANG Bao-hua QIAN Zhong-ming DUAN Xiang-lin*
Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (5) : 656-662.
Effect of lipopolysaccharide on the iron metabolism in macrophages
Objective To study the effect of lipopolysaccharide(LPS) on the activity of primary cultured macrophages and the distribution of divalent metal transporter 1 (DMT1) and ferroportin 1 (FPN1). Methods Primary cell culture, MTT chromotest, cytochemistry chromotest and cell immunofluorescence techniques were used in this work.
Results DMT1 was mainly distributed in the cytoplasm, which illuminates that DMT1 mediates the macrophage intracellular transit of iron from phagolysosome to cytoplasm. FPN1 was distributed in the cytoplasm and membrane, and the cytoplasm was the main site of FPN1 distribution in macrophages. Conclusion Iron liberation from heme inside the phagolysosome occurs after erythrophagocytosis and it is possible that FPN1 mediates intracellular transit of iron released by heme catabolism. The study found that LPS promoted the cell growth and this effect was reached to the peak in the 10-5 μg/L LPS group, but the cell growth was blocked with the increase of LPS concentration.
Divalent metal transporter 1 / Ferroportin 1 / Primary culture / Macrophage / Mouse
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