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Comparisons of expression of phosphorylated extracellular signal-regulated kinase in different levels of anterior cingulate cortex in the rat with neuropathic pain
FANG Fang SHAO Xiao-mei SHEN Zui SUN Jing FANG Jian-qiao*
Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (5) : 610-615.
Comparisons of expression of phosphorylated extracellular signal-regulated kinase in different levels of anterior cingulate cortex in the rat with neuropathic pain
Objective To observe the distribution features of phosphorylated p44/42 extracellular signal-regulated kinase(p-ERK) induced by affective pain in the anterior cingulate cortex (ACC) of the rat. Methods Twelve male SD rats were randomly divided into the control group (n=6) and spinal nerve ligation (SNL) group (n=6). The rats of the SNL group were exposed to unilateral L5 spinal nerve ligation (SNL) surgery. The behavior was examined by mechanical pain thresholds (MPTs) and the pain affect was measured using open-field test, elevated zero maze. The expressions of p-ERK in ACC (AP 3.2,2.7,2.2mm from Bregma) were detected with immunofluorescence test. Results In comparison with the control group, the unilateral mechanical pain threshold in the SNL group decreased significantly after operation. The operation also resulted in pronounced anxiety-like behavior. The p-ERK labelled cells in different levels of ACC(AP 3.2,2.7,2.2mm from Bregma) of SNL rats were (11.89±2.57),(32±4.67 ) and (17.56±2.04 ), respectively, while the control group rats were (12.44±2.16),(10±0.87 ) and (10.11±1.36). These data indicated that compared with the control group, the expressions of p-ERK in ACC(AP 2.7,2.2mm from Bregma) increased significantly in the SNL group after operation(P<0.01), besides for the level of ACC(AP 3.2mm from Bregma) (P>0.05). Conclusion The results suggest that the neuropathic pain can result in anxiety-like behavior and increased expressions of p-ERK in ACC of the rat. This change may be closely related to the expressions of p-ERK in ACC(AP 2.7，2.2mm from Bregma), but not to the level of ACC(AP 3.2mm from Bregma).
Neuropathic pain / Extracellular signal-regulated kinase / Anterior cingulate cortex / Pain related anxiety / Immunofluorescence / Rat
［1］Thibodeau MA, Welch PG, Katz J, et al. Pain-related anxiety influences pain perception differently in men and women: a quantitative sensory test across thermal pain modalities［J］.Pain,2013,154(3):419-426.
［2］Chen FL, Dong YL, Zhang ZJ, et al. Activation of astrocytes in the anterior cingulate cortex contributes to the affective component of pain in an inflammatory pain model ［J］.Brain Res Bull,2012,87(1):60-66.
［3］Crombez G, Vlaeyen JW, Heuts PH, et al. Pain-related fear is more disabling than pain -itself: evidence on the role of painrelated fear in chronic back pain disability.［J］.Pain,1999,80(1-2):329-339.
［4］Vase L, Baram S, Takakura N, et al. Specifying the nonspecific components of acupuncture analgesia［J］.Pain,2013,154(9): 1659-1667.
［5］Price DD. Psychological and neural mechanisms of the affective dimension of pain［J］.Science, 2000,288(5472):1769-1772.
［6］Cao H, Gao YJ, Ren WH, et al. Activation of extracellular signal-regulated kinase in the anterior cingulate cortex contributes to the induction and expression of affective pain［J］. J Neurosci, 2009, 29(10): 3307-3321.
［7］Gao YJ. Neural mechanisms underlying anterior cingulate cortex mediating pain aversive emotion in rats［D］.Shanghai:Institute of Neurobiology of Fudan University,2004:1-130.(in Chinese)
高永静.大鼠前扣带皮层参与痛厌恶情绪的神经机制研究［D］.上海：复旦大学神经生物学研究所,2004:1-130.
［8］Li TT, Ren WH, Xiao X, et al. NMDA NR2A and NR2B receptors in the rostral anterior cingulate cortex contribute to pain-related aversion in male rats［J］. Pain, 2009,146(1-2): 183-193.
［9］Kim SH, Chung JM. An experimental model for peripheral neuropathy produced by segmental spinal nerve ligation in the rat［J］.Pain,1992, 50(3):355-363.
［10］Yan N, Cao B, Xu J, et al. Glutamatergic activation of anterior cingulate cortex mediates the affective component of visceral pain memory in rats ［J］. Neurobiol Learn Mem,2012,97(1): 156-164.
［11］Padi SS, Kulkarni SK. Minocycline prevents the development of neuropathic pain, but not acute pain: possible anti-inflammatory and antioxidant mechanisms［J］. Eur J Pharmacol, 2008,601(1-3):79-87.
［12］Katzman MA, Pawluk EJ, Tsirgielis D, et al. Beyond chronic pain: How best to treat psychological comorbidities［J］. J Fam Pract,2014,63(5):260-264.
［13］LaGraize SC, Labuda CJ, Rutledge MA, et al. Differential effect of anterior cingulate cortex lesion on mechanical hypersensitivity and escape/avoidance behavior in an animal model of neuropathic pain［J］. Exp Neurol,2004, 188(1): 139-148.
［14］Narita M, Kuzumaki N, Narita M, et al. Chronic pain-induced emotional dysfunction is associated with astrogliosis due to cortical delta-opioid receptor dysfunction［J］. J Neurochem, 2006,97(5): 1369-1378.
［15］Matsuzawa-Yanagida K, Narita M, Nakajima M, et al.Usefulness of antidepressants for improving the neuropathic pain-like state and pain-induced anxiety through actions at different brain sites［J］. Neuropsychopharmacology,2008,33(8): 1952-1965.
［16］Zhong XL, Wei R, Zhou P, et al. Activation of anterior cingulate cortex extracellular signal-regulated kinase-1 and-2 (ERK1/2) regulates acetic acid-induced, pain-related anxiety in adult female mice［J］. Acta Histochem Cytochem,2012,45(4): 219-225.
［17］Shepherd JK, Grewal SS, Fletcher A, et al.Behavioural and pharmacological characterisation of the elevated “zero-maze” as an animal model of anxiety［J］.Psychopharmacology,1994,116(1):56-64.
［18］Lei LG, Zhang YQ, Zhao ZQ. Pain-related aversion and Fos expression in the central nervous system in rats［J］.Neuroreport,2004,15(1):67-71.
［19］Foltz EL,White LE. Pain relief by frontal cingulumotomy［J］.J Neurosurg, 1962,19:89-100.
［20］Moisset X, Bouhassira D. Brain imaging of neuropathic pain［J］. Neuroimage, 2007,37(Suppl 1):S80-88.
［21］Dai RP, Li CQ, Zhang JW, et al. Biphasic activation of extracellular signal-regulated kinase in anterior cingulate cortex distinctly regulates the development of pain-related anxiety and mechanical hypersensitivity in rats after incision［J］. Anesthesiology,2011,115(3):604-613.
［22］Han M, Xiao X, Yang Y, et al.SIP30 is required for neuropathic pain-evoked aversion in rats［J］.J Neurosci,2014,34(2):346-355.
［23］Vogt BA. Pain and emotion interactions in subregions of the cingulate gyrus［J］. Nat Rev Neurosci, 2005, 6(7):533-544.
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