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In situhybridization and analysis of core sequence of follicle-stimulating hormone and its receptor in rats submaxillary
YU Hui*LIU Gui-yun
Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (4) : 569-572.
In situhybridization and analysis of core sequence of follicle-stimulating hormone and its receptor in rats submaxillary
Objective To explore if the expression of follicle-stimulating hormone (FSH) and its receptor (FSHR) exist in the rat submandibular gland tissue, which may provide a theoretic evidence for further studying on adjusting function of the FSH in submaxillary gland. Methods Twenty normal SD rats, with a mean body weight of (200±20)g, were adopted to collect out the submandibular gland after abdominal cavity anesthesia. The method ofin situ hybridization was employed on cellular localization of FSH and FSHR. RT-PCR was used to detect if FSH and its receptor mRNA exist in the rat submandibular gland. RNA was extracted from the submandibular gland, then FSH and the core cDNA of its receptor gene were obtained with reverse transcription polymerase chain reaction and analyzed on its sequence. Results The epithelial cells of serous acinus and granular convoluted tubule in submaxillary gland of rats had FSH and FSHR mRNA hybridized signals, which were detected in cytoplasm with negative nuclei. Specific bands of FSH cDNA and FSHR cDNA were identical to be 193bp and 413bp respectively. Conclusion FSH and FSHR can be synthesized by the epithelial cells of serous acinus and granular convoluted tubule in submaxillary gland of rats, thus this study further proves that the submandibular gland is the target organ of FSH.
Follicle-stimulating hormone / Follicle-stimulating hormone receptor / Submaxillary gland / In situ hybridization / RT-PCR / Rat
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Study on follicle-stimulating hormone on the expression and function of the digestive system
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