Expression of neurofilament protein and synaptophysin in the developing spinal cord of human embryo

ZHANG Yong  LIU Xue-hong*

doi:10.3969/j.issn.0529-1356.2014.04.019

Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (4) : 545-549. DOI: 10.3969/j.issn.0529-1356.2014.04.019

Expression of neurofilament protein and synaptophysin in the developing spinal cord of human embryo

ZHANG Yong LIU Xue-hong*

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Abstract

Objective To investigate the neurofilament protein (NF) and synaptophysin(SYN) distribution in the developing stage of human embryonic spinal cord and their clinical significance. Methods Totally 16 of human embryos, aged from 2 to 4 months were used in this study. Immunohistochemical staining was uesd to detect the expression and distribution of NF and SYN in the spinal cord. Results At the second month stage, NF expression was weakly positive in only the marginal layer on both sides of the spinal cord. At 3-4 months, NF expression was positive or strong positive in the spinal cord center crack before and after the middle of partition and cortex. In the ependymal layer of the lateral part of the expression of NF positive nerve fibers were seen in the gray matter. From 2 to 4 month-age, all visible SYN positive expressions distributed in the spinal cord. With the increase of gestational age, SYN positive expression in the spinal cord staining intensity value was increased first, and then decreased. The number of positive values increased month by month. Conclusion NF and SYN both take part in the regulation of human embryonic spinal cord tissue growth.

Key words

Embryo / Spinal cord / Neurofilament protein / Synaptophysin / Immunohistochemistry / Human

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ZHANG Yong LIU Xue-hong*. Expression of neurofilament protein and synaptophysin in the developing spinal cord of human embryo[J]. Acta Anatomica Sinica. 2014, 45(4): 545-549 https://doi.org/10.3969/j.issn.0529-1356.2014.04.019

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