Welcome to visit Acta Anatomica Sinica! Today is
Chinese
Histochemical staining techniques applied in ultrathin sheet plastination slice
ZHANG Bao-ying LIANG Liang QIAO Ming-liang ZHANG Meng-nan WU Yan* ZHANG Ming*
Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (3) : 416-420.
Histochemical staining techniques applied in ultrathin sheet plastination slice
Objective To investigate whether and what staining techniques are applied to the ultrathin sheet plastination slice and whether the stained specimen is of autofluorescences. Methods A cadaveric hand block was plastinated and then sectioned as a series of 300-400 μm thick transverse sections. A total of 56 slices in total. Alternative sections were stained with hematoxylin -eosin staining (HE), Verhoeff -Van Gieson staining(VVG) or methylene blue and azureⅡstaining(MA). The stained slices were examined under a light microscope and a confocal microscope. Results The plastinated slices were stained with the three staining methods. HE staining revealed the muscle and connective tissues were red or violet, bone was violet or blue; VVG staining showed the elastic fibers was black, the collagen was red, and other tissues were yellow. MA staining showed the tendon was violet, the bone was pink, cartilage was violet, and other tissues were purple. However, the intracellular structures appeared not very well stained. The collagen, elastin and muscular structures in the stained slices were observed under a confocal microscope. Conclusion The commonly used histology staining methods can be used to stain the ultrathin sheet plastination slices. The staining provides a better observation of various tissues in the slice than the unstained slice. After staining, those autofluorescent structures in the plastinated slice are detectable under a confocal microscope.
Ultrathin sheet plastination / HE staining / Verhoeff-Van Gieson staining / Methylene blue and azureⅡ staining / Human
［1］Diao Y, Liang L, Yu C, et al. Is there an identifiable intact medial wall of the cavernous sinus? Macro and microscopic anatomical study using sheet plastination［J］. Neurosurgery, 2013, 73(1): 106-109.
［2］Liu M, Chen S, Liang L et al. Microcomputed tomography visualization of the cricoarytenoid joint cavity in cadavers［J］. J Voice, 2013,27(6):778-785.
［3］Zhang ShX, Liu Zh J, He GCh. 3D thin sectional anatomy of sella turcica region with plastination［J］. Acta anatomica sinica, 1998,29(4):337-341.(in Chinese)
张绍祥, 刘正津, 何光篪. 蝶鞍区塑化薄片断层解剖学研究［J］. 解剖学报, 1998, 29（4）：337-341.
［4］Zhang BY, Liang L, Liu M, et al. Comparison between the Biodur and PlastMat epoxy mixture［J］. Chinese Journal of Anatomy, 2013, 36(5):948-951. (in Chinese)
张宝营,梁亮,刘明,等.薄层塑化技术要点和进口与国产环氧生物塑化材料的比较［J］.解剖学杂志,2013, 36(5):948-951.
［5］Sora MC, Cook P. Epoxy plastination of biological tissue: E12 technique［J］. J Int Soc Plastination, 2007, 22(1): 31-39.
［6］Sora MC. Epoxy plastination of biological tissue: E12 ultra-thin technique［J］. J Int Soc Plastination, 2007, 22(1): 40-45.
［7］Helga F. Staining of different tissues in thick epoxy resin-impregnated sections of human fetuses［J］.Stain Technology,1989,64(2): 75-79.
［8］Phillips M, Nash L, Barnett R, et al. The use of confocal microscopy for the examination of E12 sheet plastinated human tissue［J］. J Int Soc Plastination, 2002,17(1): 12-16.
［9］Nash L, Nicholson H, Lee AS, et al. Configuration of the connective tissue in the posterior atlanto-occipital interspace: a sheet plastination and confocal microscopy study［J］. Spine, 2005, 30(12): 1359-1366.
Minimally-invasive surgical anatomy of the lateral part of the middle cranial fossa
/
| 〈 |
|
〉 |
