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Effects of Cdc20 mutation on growth of mouse embryonic fibroblast
FENG Ju-ling ZHAO Lei XIE Juan MO Ming-shu GUI Qing-jun YOU Yong ZHONG Hui WANG Li-sheng*
Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (3) : 383-387.
Effects of Cdc20 mutation on growth of mouse embryonic fibroblast
Objective Investigation of biological characteristics of Cdc20A AA/+APC min/+mouse embryonic fibroblast(MEFs) indicate the effect of Cdc20AAA/+on growth of mouse embryonic fibroblast and the possible mechanism. Methods MEFs of Cdc20AAA/+APCmin/+, Cdc20 AAA/+ ,APCmin/+and WT genotype were harvested from embryos for analysis. The growth characteristics of Cdc20 AAA/+APC min/+ , Cdc20 AAA/+ ,APC min/+and WT mouse embryonic fibroblast were analyzed through growth curve analysis and foci formation assay. Separation of sister chromatid and the presence of aneuploid were detected by karyotype analysis. Results Cell proliferation assays showed that Cdc20 AAA/+APC min/+cells grew at an accelerated rate compared with APC min/+MEFs（P＜0.01）. Foci formation assay showed that the clone forming ability was significantly increased.Cdc20 AAA/+APC min/+ MEFs showed a significant increase in the frequency of aneuploid compared with WT MEFs, which had a karyotype of 38 and contained prematurely separated sister chromatids. Conclusion Cdc20 carrying a null allele (Cdc20 AAA/+ ) may accelerate the growth and proliferation of APC min/+MEFs and present the growth characteristics of the tumor cells. The possible mechanism may be associated with chromosome instability.
Cdc20 / APCmin/+ / Embryonic fibroblast / Foci formation assay / Karyotype analysis / Chromosome / Instability / Mouse
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