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Inhibitory effect of melatonin on murine foregastric carcinoma cells via membrane receptors MT2
XU Li JIN Qing-dong GONG Xi LIU Hui ZHOU Rui-xiang*
Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (3) : 344-349.
Inhibitory effect of melatonin on murine foregastric carcinoma cells via membrane receptors MT2
Objective To investigate the inhibitory effect of melatonin on the proliferation activity of murine foregastic carcinomac (MFC) cells via melatonin membrane receptors MT2 and its relationship with the signaling pathways of mitogen-activated protein kinases (MAPKs), phosphatidylinositol 3-kinase(PI3K)-Akt. Methods Using siRNA technology to silence MT2 expression, we examined the ability of melatonin to inhibit the proliferation activity of MFC cells and its influence on the phosphorylation of ERK1/2 and Akt. Results We found two interesting effects of SiRNA-mediated silencing of MT2 expression. Firstly, it significantly antagonized the inhibitory effect of melatonin on the proliferation activity of MFC cells. Secondly, it partially blocked the inhibitory effect of melatonin on the phosphorylation of ERK1/2 and Akt. Conclusion Our results suggest that melatonin can inhibit the phosphorylation of ERK1/2 and Akt via MT2 receptors, thereby inhibiting the proliferation of gastric cancer cells.
Melatonin / Murine foregastic carcinoma cell / Melatonin membrane receptor MT2 / Akt / ERK1/2 / Real-time PCR / Mouse
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