Impact on the dopaminergic neurons of olfactory bulb in cynomolgus monkeys damaged by MPTP

CHENG Wei  REN Zhen-hua  GUAN Yun-qian  WU Di  YUE Feng  LI Guang-wu*

doi:10.3969/j.issn.0529-1356.2014.03.007

Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (3) : 333-337. DOI: 10.3969/j.issn.0529-1356.2014.03.007

Impact on the dopaminergic neurons of olfactory bulb in cynomolgus monkeys damaged by MPTP

CHENG Wei¹ REN Zhen-hua ^{1, 2} GUAN Yun-qian² WU Di² YUE Feng³ LI Guang-wu ^1*

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Abstract

Objective To explore the localization and expression of dopaminergic neurons in olfactory bulb of cynomolgus monkeys damaged by 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine（MPTP）. Methods Three adult cynomolgus monkeys were injected with MPTP to induce the damage of dopamine neurons (MPTP group) and three adult cynomolgus monkeys were as a control group. Immunohistochemical staining was performed to examine the localization and expression of dopaminergic neurons in the olfactory bulb in normal and MPTP group monkeys. The numbers of DA-positive and DARPP32-positive cells were counted and the average absorbance was measured in normal and MPTP group. Results DA and DARPP32 positive neurons were concentrated in the glomerular layer (GL) of olfactory bulb. DA positive nerve fibers were distributed in the GL while DARPP32 positive nerve fibers appeared in all layers, and most nerve fibers were in GL and external plexiform layers (EPL). After MPTP injury, compared with the normal control group, DA and DARPP32 positive neurons and nerve fibers decreased in MPTP group and DA neurons and nerve fibers decreased significantly. Conclusions DA neurons and nerve fibers are in the GL of cynomolgus monkey olfactory bulb. DA neurons and fibers are significantly reduced in the olfactory bulb of cynomolgus monkeys damaged by MPTP, which may be associated with the dysosmia in Parkinson’s disease.

Key words

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / Olfactory bulb / Dopamine / Dopamine and adenosine 3‘5‘-monophosphate-regulated phosphoprotein / Immunohistochemistry / Cynomolgus monkey

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CHENG Wei REN Zhen-hua GUAN Yun-qian WU Di YUE Feng LI Guang-wu*. Impact on the dopaminergic neurons of olfactory bulb in cynomolgus monkeys damaged by MPTP[J]. Acta Anatomica Sinica. 2014, 45(3): 333-337 https://doi.org/10.3969/j.issn.0529-1356.2014.03.007

References

［1］Doty RL. Olfaction in Parkinson’s disease and related disorders［J］. Neurobiol Dis, 2012, 46(3): 527-552.
［2］Wallace VC, Chaudhuri KR. Unexplained lower limb pain in Parkinson’s disease: a phenotypic variant of “painful Parkinson’s disease”［J］. Parkinsonism Related Disord, 2014,20(1):122-124.
［3］Neikrug AB, Maglione JE, Liu L,et al. Effects of sleep disorders on the non-motor symptoms of Parkinson disease［J］.J Clin Sleep Med,2013,9(11):1119-1129.
［4］Brodoehl S, Klingner C, Volk GF, et al. Decreased olfactory bulb volume in idiopathic Parkinson’s disease detected by 3.0-Tesla magnetic resonance imaging［J］. Mov Disord, 2012, 27(8): 1019-1025.
［5］Haehner A, Boesveldt S, Berendse HW. et al. Prevalence of smell loss in Parkinson’s disease-a multicenter study［J］. Parkinsonism Relat Disord, 2009, 15(7): 490-494.
［6］Belzunegui S, Sebastián W, Garrido-Gil P, et al. The number of dopaminergic cells is increased in the olfactory bulb of monkeys chronically exposed to MPTP［J］. Synapse, 2007, 61(12): 1006-1012.
［7］Bundschuh ST, Zhu P, Schrer YP, et al. Dopaminergic modulation of mitral cells and odor responses in the zebrafish olfactory bulb［J］. J Neurosci, 2012, 32(20): 6830-6840.
［8］Cave J W, Baker H. Dopamine Systems in the Forebrain. Development and Engineering of Dopamine Neurons ［M］. New York: Springer, 2009: 15-35.
［9］Tissingh G, Berendse HW, Bergmans P, et al. Loss of olfaction in de novo and treated Parkinson’s disease: possible implications for early diagnosis［J］. Mov Disord, 2001; 16(1): 41-46.
［10］Huisman E, Uyings HB, Hoogland PV. A 100% increase of dopaminergic in Parkinson’s disease［J］. Mov Disord, 2004, 19(6): 687-692.
［11］Herring B, Shchulze S, Reichman H, et al. A longitudinal study of olfactory function in patients with idiopathic Parkinson’s diease［J］. J Neurol, 2008, 255(3): 367-370.
［12］H?kfelt T, Halasz N, Ljungdahl ?, et al. Histochemical support for a dopaminergic mechanism in the dendrites of certain periglomerular cells in the rat olfactory bulb ［J］. Neurosci Lett, 1975, 1(2): 85-90.
［13］Yuste JE, Echeverry MB, Ros-Bernal F, et al. 7-Nitroindazole down-regulates dopamine/DARPP-32 signaling in neostriatal neurons in a rat model of Parkinson’s disease［J］. Neuropharmacology, 2012,63(7): 1258-1267.
［14］Brown AM, Baucum AJ, Bass MA, et al. Association of protein phosphatase 1 gamma 1 with spinophilin suppresses phosphatase activity in a Parkinson disease model［J］. J Biol Chem, 2008, 283(21): 14286-14294.